Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-amino-4-hydroxypterin | substrate inhibition above 0.01 mM | Neurospora crassa | |
2-Iodosobenzoic acid | effective inhibition of xanthine and pterine oxidation | Neurospora crassa | |
4-hydroxymercuribenzoate | effective inhibition of xanthine and pterine oxidation | Neurospora crassa | |
4-hydroxypyrazolo(3,4-d)pyrimidine | i.e. allopurinol; inhibition of pterine oxidation at 0.0003 mM | Neurospora crassa | |
adenine | effective inhibition of xanthine oxidation at 0.001 mM | Neurospora crassa | |
arsenite | inhibition of xanthine or pterine oxidation at 0.3 mM, diaphorase activity unaffected | Neurospora crassa | |
guanine | effective inhibition of xanthine oxidation at 0.001 mM | Neurospora crassa | |
KCN | 35% inhibition of pterine oxidation, 60% inhibition of diaphorase activity at 5 mM | Neurospora crassa | |
NaN3 | slight inhibition of dehydrogenase activity | Neurospora crassa | |
o-phenanthroline | 50% inhibition at 5-15 mM | Neurospora crassa | |
Thiourea | 65% inhibition of xanthine dehydrogenase activity at 20 mM | Neurospora crassa | |
Urate | inhibition of xanthine oxidation at 0.06 mM | Neurospora crassa | |
xanthine | substrate inhibition above 0.13 mM | Neurospora crassa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0022 | - |
NAD+ | oxidation of 2-amino-4-hydroxy-pterin | Neurospora crassa | |
0.0035 | - |
2-amino-4-hydroxypterin | - |
Neurospora crassa | |
0.0082 | - |
xanthine | xanthine dehydrogenase activity | Neurospora crassa | |
0.021 | - |
hypoxanthine | xanthine dehydrogenase activity | Neurospora crassa | |
0.021 | - |
2,6-dichloroindophenol | diaphorase activity | Neurospora crassa | |
0.028 | - |
NAD+ | xanthine dehydrogenase activity | Neurospora crassa | |
6 | - |
NADH | diaphorase activity | Neurospora crassa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | 12.1 mol iron per mol enzyme | Neurospora crassa | |
Mo | 1 mol molybdenum per mol enzyme | Neurospora crassa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
155000 | - |
2 * 155000, SDS-PAGE | Neurospora crassa |
357000 | - |
sucrose density gradient centrifugation | Neurospora crassa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-amino-4-hydroxy-pterin + NAD+ + H2O | Neurospora crassa | i.e. pterin | isoxanthopterin + NADH | - |
r | |
hypoxanthine + NAD+ + H+ + O2- + H2O | Neurospora crassa | - |
xanthine + NADH + H2O2 | - |
r | |
xanthine + NAD+ + H2O | Neurospora crassa | degradative pathway of conversion of purines to ammonia | urate + NADH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neurospora crassa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, immunoabsorption chromatography | Neurospora crassa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | inducible | Neurospora crassa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.152 | - |
- |
Neurospora crassa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,6-dichloroindophenol + NADH + H+ | - |
Neurospora crassa | reduced 2,6-dichlorophenolindophenol + NAD+ | - |
r | |
2-amino-4-hydroxy-pterin + NAD+ + H2O | i.e. pterin | Neurospora crassa | isoxanthopterin + NADH | - |
r | |
2-amino-4-hydroxypterin + NAD+ + H2O | i.e. pterin | Neurospora crassa | isoxanthopterin + NADH | - |
r | |
hypoxanthine + NAD+ + H+ + O2- + H2O | - |
Neurospora crassa | xanthine + NADH + H2O2 | - |
r | |
NADH + electron acceptor + H2O | 2,6-dichloroindophenol as electron acceptor | Neurospora crassa | NAD+ + reduced electron acceptor | - |
? | |
xanthine + NAD+ + H2O | degradative pathway of conversion of purines to ammonia | Neurospora crassa | urate + NADH | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 155000, SDS-PAGE | Neurospora crassa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | 2 mol per mol enzyme | Neurospora crassa | |
additional information | non-heme flavoprotein | Neurospora crassa | |
NAD+ | cannot be replaced by NADP+ | Neurospora crassa | |
NADH | - |
Neurospora crassa |