Activating Compound | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzoate | increases total activity up to 0.005 mM | Rattus sp. |
Application | Comment | Organism |
---|---|---|
medicine | modification of xanthine oxidoreductase by disulfiram that is used in the management of chronic alcolism | Rattus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-Iodosobenzoic acid | 50% inhibition at 0.0025 mM of NAD+-dependent activity by enzyme inactivation, not by conversion to the O2-dependent activity | Rattus sp. | |
4-chloromercuribenzoate | decreases NAD+-dependent activity from 0.01 up to 0.05 mM with simultaneous inactivation of the enzyme | Rattus sp. | |
5,5-dithiobis-(2-nitrobenzoate) | conversion from of NAD+-dependent to O2-dependent activity without any effect on the total activity | Rattus sp. | |
NADH | product inhibition | Rattus sp. | |
tetraethyldithiodicarbonic diamide | i.e. disulfiram; transformation of the NAD+- to the O2-dependent activity up to 0.025 mM, up to 80% loss of NAD+-dependent activity, modification of one thiol group in the active centre, NAD+ protects against modification due to a single thiol group involved in NAD+-binding within the active centre | Rattus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
xanthine + NAD+ + H2O | Rattus sp. | NAD+-dependent form is postulated to play a regulatory role in purine metabolism | urate + NADH | - |
? | |
xanthine + O2 + H2O | Rattus sp. | NAD+-independent xanthine oxidase activity, low activity present in the enzyme preparation, conversion of the NAD+-dependent to NAD+-independent activity by some thiol reagents | urate + O2- + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, mainly NAD+-dependent activity | Rattus sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus sp. | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
xanthine + NAD+ + H2O | NAD+-dependent form is postulated to play a regulatory role in purine metabolism | Rattus sp. | urate + NADH | - |
? | |
xanthine + O2 + H2O | NAD+-independent xanthine oxidase activity, low activity present in the enzyme preparation, conversion of the NAD+-dependent to NAD+-independent activity by some thiol reagents | Rattus sp. | urate + O2- + 2 H+ | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Rattus sp. |