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Literature summary for 1.17.1.4 extracted from

  • Doyle, W.A.; Burke, J.F.; Chovnick, A.; Dutton, F.L.; Whittle, J.R.S.; Bray, R.C.
    Properties of xanthine dehydrogenase variants from rosy mutant strains of Drosophila melanogaster and their relevance to the enzyme's structure and mechanism (1996), Eur. J. Biochem., 239, 782-795.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
E89K
-
Drosophila melanogaster
G1011E within the molybdenum domain, no activity without oxidative activation Drosophila melanogaster
G353D modifications to the NAD+-NADH-binding sites Drosophila melanogaster
S357F modifications to the NAD+-NADH-binding sites Drosophila melanogaster

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
NAD+ electron donor: pterin Drosophila melanogaster
0.009
-
NADH electron acceptor: 2,6-dichlorophenolindophenol Drosophila melanogaster
0.029
-
xanthine electron acceptors: phenazine methosulfate/cytochrome c Drosophila melanogaster
0.033
-
NAD+ increased Km for mutant G353D, electron acceptor: pterin Drosophila melanogaster
0.048
-
NADH increased Km for mutant G353D, electron acceptor: 2,6-dichlorophenolindophenol Drosophila melanogaster

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-amino-4-hydroxy-pterin + NAD+ + H2O Drosophila melanogaster i.e. pterin isoxanthopterin + NADH
-
?
xanthine + NAD+ + H2O Drosophila melanogaster
-
urate + NADH
-
?
xanthine + O2 + H2O Drosophila melanogaster
-
urate + O2- + 2 H+
-
?

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques Drosophila melanogaster

Source Tissue

Source Tissue Comment Organism Textmining
whole body
-
Drosophila melanogaster
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-amino-4-hydroxy-pterin + methylene blue + H2O
-
Drosophila melanogaster isoxanthopterin + reduced methylene blue
-
?
2-amino-4-hydroxy-pterin + NAD+ + H2O i.e. pterin Drosophila melanogaster isoxanthopterin + NADH
-
?
2-amino-4-hydroxypterin + NAD+ + H2O i.e. pterin Drosophila melanogaster isoxanthopterin + NADH
-
?
NADH + electron acceptor + H2O
-
Drosophila melanogaster NAD+ + reduced electron acceptor
-
?
xanthine + 2,6-dichlorophenolindophenol + H2O
-
Drosophila melanogaster urate + ?
-
?
xanthine + methylene blue + H2O
-
Drosophila melanogaster urate + reduced methylene blue
-
?
xanthine + NAD+ + H2O
-
Drosophila melanogaster urate + NADH
-
?
xanthine + O2 + H2O
-
Drosophila melanogaster urate + O2- + 2 H+
-
?
xanthine + phenazine methosulfate + cytochrome c + H2O
-
Drosophila melanogaster urate + ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
17.1
-
NADH activation of mutant G1011E activity after preincubation with phenazine methosulfate and cytochrome c, electron acceptor: methylene blue Drosophila melanogaster
22.8
-
xanthine mutant E89K, electron acceptor: NAD+ Drosophila melanogaster
24.9
-
xanthine electron acceptor: NAD+ Drosophila melanogaster
29
-
NADH activation of mutant G1011E activity after preincubation with ferricyanide, electron acceptor: methylene blue Drosophila melanogaster