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Literature summary for 1.16.3.2 extracted from

  • Hudson, A.; Andrews, S.; Hawkins, C.; Williams, J.; Izuhara, M.; Meldrum, F.; Mann, S.; Harrison, P.; Guest, J.
    Overproduction, purification and characterization of the Escherichia coli ferritin (1993), Eur. J. Biochem., 218, 985-995.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JRG2157 cells Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
microdialysis against 20 mM piperazine/HCl (pH 5.2) Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Iron the non-heme enzyme contains 5-20 non-heme iron atoms per holomer Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19400
-
24 * 19400, SDS-PAGE Escherichia coli
19424
-
24 * 19424, calculated from amino acid sequence Escherichia coli
465000
-
gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Fe(II) + O2 + H2O Escherichia coli
-
[FeO(OH)] + H+ + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A998
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Q-Sepharose column chromatography, and Sephacryl S300 gel filtration Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Fe(II) + O2 + H2O
-
Escherichia coli [FeO(OH)] + H+ + H2O2
-
?

Subunits

Subunits Comment Organism
24-mer 24 * 19400, SDS-PAGE Escherichia coli
24-mer 24 * 19424, calculated from amino acid sequence Escherichia coli

Synonyms

Synonyms Comment Organism
ferritin
-
Escherichia coli
Ftn
-
Escherichia coli

pI Value

Organism Comment pI Value Maximum pI Value
Escherichia coli isoelectric focusing
-
5