Cloned (Comment) | Organism |
---|---|
mutant enzymes are expressed in Saccharomyces cerevisae strain INVScI | Brassica rapa subsp. oleifera |
Protein Variants | Comment | Organism |
---|---|---|
D208R | inactive | Brassica rapa subsp. oleifera |
D210R | inactive | Brassica rapa subsp. oleifera |
F59R | the mutant shows markedly decreased conversion rate compared to the wild type enzyme | Brassica rapa subsp. oleifera |
G28R | the mutant shows slightly decreased conversion rate compared to the wild type enzyme | Brassica rapa subsp. oleifera |
G368R | inactive | Brassica rapa subsp. oleifera |
G52R | the mutant shows wild type activity | Brassica rapa subsp. oleifera |
H63R | inactive | Brassica rapa subsp. oleifera |
L369A | the mutant shows markedly decreased conversion rate compared to the wild type enzyme | Brassica rapa subsp. oleifera |
L71R | the mutant shows wild type activity | Brassica rapa subsp. oleifera |
N203R | inactive | Brassica rapa subsp. oleifera |
Q372H | the mutant shows markedly decreased conversion rate compared to the wild type enzyme | Brassica rapa subsp. oleifera |
T56R | the mutant shows slightly decreased conversion rate compared to the wild type enzyme | Brassica rapa subsp. oleifera |
W190A | the mutant shows markedly decreased conversion rate compared to the wild type enzyme | Brassica rapa subsp. oleifera |
W345A | the mutant shows markedly decreased conversion rate compared to the wild type enzyme | Brassica rapa subsp. oleifera |
Y31A | the mutant shows slightly decreased conversion rate compared to the wild type enzyme | Brassica rapa subsp. oleifera |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Brassica rapa subsp. oleifera | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(4E)-sphing-4-enine ceramide + ferrocytochrome b5 + O2 + H+ | Brassica rapa subsp. oleifera | - |
(4E,8E)-sphing-4,8-dienine ceramide + ferricytochrome b5 + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brassica rapa subsp. oleifera | G9I7G1 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(4E)-sphing-4-enine ceramide + ferrocytochrome b5 + O2 + H+ | - |
Brassica rapa subsp. oleifera | (4E,8E)-sphing-4,8-dienine ceramide + ferricytochrome b5 + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DELTA8-sphingolipid desaturase | - |
Brassica rapa subsp. oleifera |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome b5 | - |
Brassica rapa subsp. oleifera |