Application | Comment | Organism |
---|---|---|
medicine | inhibitors for P-45011beta, against aldosterone overproduction which leads to oedematous diseases and hypertension | Bos taurus |
General Stability | Organism |
---|---|
deoxycorticosterone, protection of enzyme from inactivation by 18-ethynylprogesterone | Bos taurus |
phospholipid vesicles, 0.2% soybean lecithin, added to purified P-45011beta for storage and during incubation | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
18-ethynylprogesterone | mechanism-based inhibition; progesterone analog, time-dependent pseudo-first-order inactivation, concentration dependent | Bos taurus | |
18-vinylprogesterone | mechanism-based inhibition; progesterone analog, with NADPH, time and concentration dependent, irreversible, pseudo-first-order process, covalent binding to and destruction of prosthetic heme group, 5fold more effective than its acetylenic analog 18-ethynylprogesterone | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Bos taurus | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
copurification of P-450 11beta-2 and 11beta-3, ammonium sulfate precipitation, octyl-Sepharose, separation of 2 isoforms by hydroxyapatite column connected to an FPLC system | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal cortex | - |
Bos taurus | - |
Storage Stability | Organism |
---|---|
-80°C, after octyl-Sepharose, deoxycorticosterone-bound, 50 mM potassium phosphate, pH 7.4, 0.5% sodium cholate, 0.2% Tween 20, 0.01 mM 11-deoxycorticosterone, 0.1 mM dithiothreitol, 0.1 mM EDTA | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
11-deoxycorticosterone + reduced adrenal ferredoxin + 2 H+ + 2 O2 | 3 step 11beta-/18-hydroxylation to aldosterone | Bos taurus | aldosterone + oxidized adrenal ferredoxin + 2 H2O | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | requirement during inactivation | Bos taurus | |
NADPH | adrenodoxin and NADPH-linked adrenodoxin reductase | Bos taurus |