Activating Compound | Comment | Organism | Structure |
---|---|---|---|
L-ascorbate | adding of a second electron donor, such as ascorbate, led to a 10fold increase in the heme conversion rate | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
expressed in Nicotiana benthamiana and Escherichia coli BL21 (DE3) cells | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0013 | - |
protoheme | isoform HY1, at pH 7.2 and 25°C | Arabidopsis thaliana | |
0.0027 | - |
protoheme | isoform HO3, at pH 7.2 and 25°C | Arabidopsis thaliana | |
0.0057 | - |
protoheme | isoform HO4, at pH 7.2 and 25°C | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Arabidopsis thaliana | 9507 | - |
nucleus | - |
Arabidopsis thaliana | 5634 | - |
plastid | isoforms HY1, HO3 and HO4 | Arabidopsis thaliana | 9536 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
protoheme + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ | Arabidopsis thaliana | - |
biliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Talon-cobalt-affinity column chromatography | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
protoheme + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ | - |
Arabidopsis thaliana | biliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
haem oxygenase | - |
Arabidopsis thaliana |
Ho1 | - |
Arabidopsis thaliana |
Ho3 | - |
Arabidopsis thaliana |
HO4 | - |
Arabidopsis thaliana |
HY1 | - |
Arabidopsis thaliana |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 45 | the relative enzyme activity increases between 10°C and 45°C | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
isoform HO3 | Arabidopsis thaliana |
7.3 | - |
isoforms HO4 and HY1 | Arabidopsis thaliana |