Cloned (Comment) | Organism |
---|---|
gene MdF3'HI, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, functional ectopic expression in Arabidopsis thaliana tt7 mutant and in Nicotiana tabacum | Malus domestica |
gene MdF3'HIIa, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, functional ectopic expression in Arabidopsis thaliana tt7 mutant and in Nicotiana tabacum | Malus domestica |
gene MdF3'HIIb, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, functional ectopic expression in Arabidopsis thaliana tt7 mutant and in Nicotiana tabacum | Malus domestica |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Malus domestica | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydrokaempferol + [reduced NADPH-hemoprotein reductase] + O2 | Malus domestica | - |
dihydroquercetin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
kaempferol + [reduced NADPH-hemoprotein reductase] + O2 | Malus domestica | - |
quercetin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
naringenin + [reduced NADPH-hemoprotein reductase] + O2 | Malus domestica | - |
eriodictyol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Malus domestica | C5IGQ3 | gene MdF3'HI; gene MdF3'HI | - |
Malus domestica | C5IGQ4 | gene MdF3'HIIa; gene MdF3'HIIa | - |
Malus domestica | C5IGQ5 | gene MdF3'HIIb; gene MdF3'HIIb | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | throughout the development of fruits, the transcriptional levels of MdF3'H genes along with other anthocyanin biosynthesis genes are higher in the red-skinned cv. Red Delicious than that in the yellow-skinned cv. Golden Delicious. Patterns of MdF3'H gene expression correspond to accumulation patterns of flavonoids in apple fruit | Malus domestica | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydrokaempferol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Malus domestica | dihydroquercetin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
kaempferol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Malus domestica | quercetin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
naringenin + [reduced NADPH-hemoprotein reductase] + O2 | - |
Malus domestica | eriodictyol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
F3'H | - |
Malus domestica |
flavonoid 3'-hydroxylase | - |
Malus domestica |
More | F3'H is a microsomal cytochrome P450-dependent monooxygenase | Malus domestica |
Organism | Comment | Expression |
---|---|---|
Malus domestica | ectopic expression of apple F3'H genes contributes to anthocyanin accumulation in the Arabidopsis thaliana transparent testa7-1 mutant tt7 grown under nitrogen stress, overview. Transgenic Arabidopsis tt7 seedlings xpressing apple F3'H regain red color pigmentation and significantly accumulate both 4'-hydroxylated pelargonidin and 3',4'-hydroxylated cyanidin | additional information |
General Information | Comment | Organism |
---|---|---|
evolution | duplication of F3'H genes in plants, overview | Malus domestica |
metabolism | MdF3'H genes are coordinately expressed with other genes in the anthocyanin biosynthetic pathway in apple | Malus domestica |
physiological function | the enzyme introduces a hydroxyl group at 3' position of the B-ring of the flavonoid molecule leading to the formation of 3',4'-hydroxylated flavonoids | Malus domestica |