Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Candida albicans |
truncated HO-1 lacking the 23 C-terminal residues, truncated HO-2 lacking the 23 C-terminal residues in the pBAce expression vector construct transformed into Escherichia coli DH5alpha | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
benzyl isocyanide | most potent uncompetitive inhibitor with respect to heme, is the strongest ligand to ferrous heme | Candida albicans | |
benzyl isocyanide | most potent uncompetitive inhibitor with respect to heme, is the strongest ligand to ferrous heme with an almost 42fold greater binding affinity for HO-1 than isopropyl isocyanide. HO-2 displays a similar trend. Ferric verdoheme-HO-1 shows a 2fold higher affinity for the inhibitor than ferric heme-HO-1 | Homo sapiens | |
isopropyl isocyanide | - |
Candida albicans | |
isopropyl isocyanide | binding affinity is the weakest for HO-1. HO-2 displays a similar trend | Homo sapiens | |
additional information | whereas equilibrium binding of the isocyanides to ferric human heme oxygenases is rapid, binding to ferric Hmx1 is much slower | Candida albicans | |
n-butyl isocyanide | - |
Candida albicans | |
n-butyl isocyanide | displays a 9fold higher affinity than isopropyl isocyanide for HO-1. HO-2 displays a similar trend. Ferric verdoheme-HO-1 shows a 2fold higher affinity for the inhibitor than ferric heme-HO-1 | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
heme | HO-1 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida albicans | - |
- |
- |
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to apparent homogeneity | Candida albicans |
truncated HO-1 and HO-2 lacking the 23 C-terminal residues, by gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
heme + electron donor + O2 | - |
Homo sapiens | biliverdin + Fe2+ + CO + oxidized electron donor + H2O | - |
? | |
heme + electron donor + O2 | - |
Candida albicans | biliverdin + Fe2+ + CO + oxidized electron donor + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
heme oxygenase | - |
Candida albicans |
heme oxygenase-1 | - |
Homo sapiens |
Hmx1 | - |
Candida albicans |
HO-1 | - |
Homo sapiens |
HO-2 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Candida albicans | |
additional information | CO as a ferrous heme ligand or ferricyanide as an oxidant have no effect | Homo sapiens | |
NADPH | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0015 | - |
benzyl isocyanide | HO-1 | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0001 | - |
HO-2, inhibition of conversion of heme | Homo sapiens | benzyl isocyanide | |
0.00013 | - |
HO-1, inhibition of conversion of heme | Homo sapiens | benzyl isocyanide | |
0.00025 | - |
HO-1, inhibition of conversion of heme | Homo sapiens | n-butyl isocyanide | |
0.00034 | - |
HO-2, inhibition of conversion of heme | Homo sapiens | n-butyl isocyanide | |
0.00072 | - |
HO-1, inhibition of conversion of heme | Homo sapiens | isopropyl isocyanide | |
0.00117 | - |
HO-2, inhibition of conversion of heme | Homo sapiens | isopropyl isocyanide | |
0.005 | - |
HO-1, inhibition of conversion of verdoheme | Homo sapiens | benzyl isocyanide | |
0.045 | - |
HO-1, inhibition of conversion of verdoheme | Homo sapiens | n-butyl isocyanide |