General Stability | Organism |
---|---|
3-hydroxyphenylacetate protects against thermal inactivation | Flavobacterium sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-Chloromercuri-4-nitrophenol | - |
Flavobacterium sp. | |
5,5'-dithiobis(2-nitrobenzoate) | - |
Flavobacterium sp. | |
Cl- | competitive to the aromatic substrate | Flavobacterium sp. | |
NEM | - |
Flavobacterium sp. | |
p-hydroxymercuribenzoate | - |
Flavobacterium sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.079 | - |
3-hydroxyphenylacetate | - |
Flavobacterium sp. | |
0.09 | - |
3,4-Dihydroxyphenylacetate | - |
Flavobacterium sp. | |
0.14 | - |
NADPH | - |
Flavobacterium sp. | |
0.15 | - |
NADH | - |
Flavobacterium sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63000 | - |
2 * 63000, SDS-PAGE | Flavobacterium sp. |
150000 | 200000 | gel filtration | Flavobacterium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Flavobacterium sp. | - |
- |
- |
Flavobacterium sp. JS-7 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Flavobacterium sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:phenylacetic acid-grown cell | - |
Flavobacterium sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
20.1 | - |
- |
Flavobacterium sp. |
Storage Stability | Organism |
---|---|
-70°C, stable for at least 1 month | Flavobacterium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dihydroxyphenylacetate + NAD(P)H + O2 + H+ | - |
Flavobacterium sp. | 2,4,5-trihydroxyphenylacetate + NAD(P)+ + H2O | - |
? | |
3,4-dihydroxyphenylacetate + NAD(P)H + O2 + H+ | - |
Flavobacterium sp. JS-7 | 2,4,5-trihydroxyphenylacetate + NAD(P)+ + H2O | - |
? | |
3,4-Dihydroxyphenylacetate + NADH + O2 | - |
Flavobacterium sp. | 2,4,5-Trihydroxyphenylacetate + NAD+ | - |
? | |
3,4-Dihydroxyphenylacetate + NADH + O2 | - |
Flavobacterium sp. JS-7 | 2,4,5-Trihydroxyphenylacetate + NAD+ | - |
? | |
3-Hydroxyphenylacetate + NADH + O2 | NADPH and NADH are oxidized at a comparable rate | Flavobacterium sp. | 2,5-Dihydroxyphenylacetate + NAD+ | - |
? | |
3-Hydroxyphenylacetate + NADH + O2 | NADPH and NADH are oxidized at a comparable rate | Flavobacterium sp. JS-7 | 2,5-Dihydroxyphenylacetate + NAD+ | - |
? | |
3-Hydroxyphenylacetate + NADPH + O2 | NADPH and NADH are oxidized at a comparable rate | Flavobacterium sp. | 2,5-Dihydroxyphenylacetate + NADP+ | - |
? | |
3-Hydroxyphenylacetate + NADPH + O2 | NADPH and NADH are oxidized at a comparable rate | Flavobacterium sp. JS-7 | 2,5-Dihydroxyphenylacetate + NADP+ | - |
? | |
4-Hydroxyphenylacetate + NADH + O2 | - |
Flavobacterium sp. | 2,4-Dihydroxyphenylacetate + NAD+ | - |
? | |
additional information | in the absence of oxygen the free enzyme is slowly reduced by NADPH or NADH | Flavobacterium sp. | ? | - |
? | |
additional information | in the absence of oxygen the free enzyme is slowly reduced by NADPH or NADH | Flavobacterium sp. JS-7 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 63000, SDS-PAGE | Flavobacterium sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15.6 | - |
3,4-Dihydroxyphenylacetate | - |
Flavobacterium sp. | |
22.4 | - |
3-hydroxyphenylacetate | - |
Flavobacterium sp. | |
35.8 | - |
NADPH | - |
Flavobacterium sp. | |
36 | - |
NADH | - |
Flavobacterium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.3 | 8.9 | - |
Flavobacterium sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.3 | 8.9 | 50% of maximal activity at pH 7.3 and 8.9 | Flavobacterium sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | 37°C, stable | Flavobacterium sp. |
9 | - |
strong inactivation above | Flavobacterium sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | contains 1 mol non-covalently bound FAD per mol of subunit | Flavobacterium sp. |