Literature summary for 1.14.13.241 extracted from

Sparrow, L.G.; Ho, P.P.K.; Sundaram, T.K.; Zach, D.; Nyns, E.J.; Snell, E.E.
The bacterial oxidation of vitamin B6. VII. Purification, properties, and mechanism of action of an oxygenase which cleaves the 3-hydroxypyridine ring (1969), J. Biol. Chem., 244, 2590-2600.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
-	Pseudomonas sp.

General Stability

General Stability	Organism
activity is lost on resolution with acidic ammonium sulfate and can be completely restored with FAD+, but not with FMN	Pseudomonas sp.
highly sensitive to sulfhydryl reagents but not to chelating agents and is stabilized by high concentrations of mercaptoethanol and glycerol	Pseudomonas sp.

Inhibitors

Inhibitors	Comment	Organism	Structure
5-pyridoxic acid	-	Pseudomonas sp.
6-methylnicotinic acid	-	Pseudomonas sp.
p-chloromercuribenzoate	-	Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
166000	-	sedimentation equlibrium	Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
3-hydroxy-4-hydroxymethyl-2-methylpyridine-5-carboxylate + NADPH + O2 + H3O+	Pseudomonas sp.	bacterial vitamin B6 degradation pathway	2-(acetamidomethylene)-3-(hydroxymethyl)succinate + NADP+ + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas sp.	-	-	-

Oxidation Stability

Oxidation Stability	Organism
highly sensitive to oxidation	Pseudomonas sp.

Purification (Commentary)

Purification (Comment)	Organism
-	Pseudomonas sp.

Storage Stability

Storage Stability	Organism
-20°C, can be stored up to 1 month with loss about 20% of its activity in a mixture of 50% glycerol and 0.1% 2-mercaptoethanol	Pseudomonas sp.
4°C, loses activity rapidly in absence of mercaptoethanol	Pseudomonas sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3-hydroxy-4-hydroxymethyl-2-methylpyridine-5-carboxylate + NADPH + O2	-	Pseudomonas sp.	2-(acetamidomethylene)-3-(hydroxymethyl)succinate + NADP+ + H2O	-	?
3-hydroxy-4-hydroxymethyl-2-methylpyridine-5-carboxylate + NADPH + O2 + H3O+	bacterial vitamin B6 degradation pathway	Pseudomonas sp.	2-(acetamidomethylene)-3-(hydroxymethyl)succinate + NADP+ + H2O	-	?
5-pyridoxic acid + NADPH + O2 + H3O+	reaction rate 3%	Pseudomonas sp.	2-(acetylaminomethylene)succinate + NADP+ + H2O	-	?
additional information	2,6-dichloroindophenol is no substrate	Pseudomonas sp.	?	-	?

Synonyms

Synonyms	Comment	Organism
compound I oxygenase	-	Pseudomonas sp.

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	8	broad pH-optimum in phosphate, diphosphate and Tris buffer	Pseudomonas sp.

Cofactor

Cofactor	Comment	Organism	Structure
FAD	contains 2 mol of FAD+ per mol	Pseudomonas sp.

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.06	-	5-pyridoxic acid	-	Pseudomonas sp.
0.2	-	6-methylnicotinic acid	-	Pseudomonas sp.

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Release 2023.1 (January 2023)