Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
additional information | functional expression in Escherichia coli of tricistronic constructs consisting of P450cam and the auxiliary proteins, Pd and PdR. Transformed bacterial whole cells efficiently oxidize (1R)-(+)-camphor to 6-exo-hydroxycamphor and interestingly limonene to (-)-perillyl alcohol. These bioengineered Escherichia coli cells possess a heterologous selfsufficient P450 catalytic system | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-camphor + NADPH + H+ + O2 | - |
Pseudomonas putida | (+)-6-exo-hydroxycamphor + NADP+ + H2O | - |
? | |
limonene + NADPH + H+ + O2 | - |
Pseudomonas putida | (-)-perillyl alcohol + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
P450cam | - |
Pseudomonas putida |