Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Zonocerus variegatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00108 | - |
senecionine | in 100 mM glycine/NaOH buffer pH 9.0, at 37°C | Zonocerus variegatus | |
0.00983 | - |
atropine | in 100 mM glycine/NaOH buffer pH 9.0, at 37°C | Zonocerus variegatus | |
0.01193 | - |
monocrotaline | in 100 mM glycine/NaOH buffer pH 9.0, at 37°C | Zonocerus variegatus | |
0.2639 | - |
heliotrine | in 100 mM glycine/NaOH buffer pH 9.0, at 37°C | Zonocerus variegatus | |
0.8376 | - |
phalaenopsine | in 100 mM glycine/NaOH buffer pH 9.0, at 37°C | Zonocerus variegatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47793 | - |
2 * 47793, calculated from amino acid sequence | Zonocerus variegatus |
91200 | - |
gel filtration | Zonocerus variegatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
senecionine + NADPH + H+ + O2 | Zonocerus variegatus | most efficient substrate | senecionine N-oxide + NADP+ + H2O | - |
? | |
seneciphylline + NADPH + H+ + O2 | Zonocerus variegatus | second best substrate | seneciphylline N-oxide + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zonocerus variegatus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-NTA agarose column chromatography | Zonocerus variegatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
atropine + NADPH + H+ + O2 | - |
Zonocerus variegatus | atropine N-oxide + NADP+ + H2O | - |
? | |
axillaridine + NADPH + H+ + O2 | - |
Zonocerus variegatus | axillaridine N-oxide + NADP+ + H2O | - |
? | |
axillarine + NADPH + H+ + O2 | - |
Zonocerus variegatus | axillarine N-oxide + NADP+ + H2O | - |
? | |
cysteamine + NADPH + H+ + O2 | - |
Zonocerus variegatus | cysteamine N-oxide + NADP+ + H2O | - |
? | |
heliotrine + NADPH + H+ + O2 | - |
Zonocerus variegatus | heliotrine N-oxide + NADP+ + H2O | - |
? | |
indicine + NADPH + H+ + O2 | - |
Zonocerus variegatus | indicine N-oxide + NADP+ + H2O | - |
? | |
monocrotaline + NADPH + H+ + O2 | - |
Zonocerus variegatus | monocrotaline N-oxide + NADP+ + H2O | - |
? | |
additional information | no activity with senkirkine, heliotridine, supinidine, ephedrine, dimethylaniline, L-cysteine, hydroxylamine, and glutathione | Zonocerus variegatus | ? | - |
? | |
nicotine + NADPH + H+ + O2 | - |
Zonocerus variegatus | nicotine N-oxide + NADP+ + H2O | - |
? | |
phalaenopsine + NADPH + H+ + O2 | - |
Zonocerus variegatus | phalaenopsine N-oxide + NADP+ + H2O | - |
? | |
retronecine + NADPH + H+ + O2 | - |
Zonocerus variegatus | retronecine N-oxide + NADP+ + H2O | - |
? | |
retrorsine + NADPH + H+ + O2 | - |
Zonocerus variegatus | retrorsine N-oxide + NADP+ + H2O | - |
? | |
rinderine + NADPH + H+ + O2 | - |
Zonocerus variegatus | rinderine N-oxide + NADP+ + H2O | - |
? | |
sarracine + NADPH + H+ + O2 | - |
Zonocerus variegatus | sarracine N-oxide + NADP+ + H2O | - |
? | |
senecionine + NADPH + H+ + O2 | most efficient substrate | Zonocerus variegatus | senecionine N-oxide + NADP+ + H2O | - |
? | |
seneciphylline + NADPH + H+ + O2 | second best substrate | Zonocerus variegatus | seneciphylline N-oxide + NADP+ + H2O | - |
? | |
senecivernine + NADPH + H+ + O2 | - |
Zonocerus variegatus | senecivernine N-oxide + NADP+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 47793, calculated from amino acid sequence | Zonocerus variegatus |
Synonyms | Comment | Organism |
---|---|---|
PA N-oxygenase | - |
Zonocerus variegatus |
PNO | - |
Zonocerus variegatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | - |
- |
Zonocerus variegatus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
heliotrine | in 100 mM glycine/NaOH buffer pH 9.0, at 37°C | Zonocerus variegatus | |
0.13 | - |
phalaenopsine | in 100 mM glycine/NaOH buffer pH 9.0, at 37°C | Zonocerus variegatus | |
0.37 | - |
atropine | in 100 mM glycine/NaOH buffer pH 9.0, at 37°C | Zonocerus variegatus | |
0.96 | - |
monocrotaline | in 100 mM glycine/NaOH buffer pH 9.0, at 37°C | Zonocerus variegatus | |
0.99 | - |
senecionine | in 100 mM glycine/NaOH buffer pH 9.0, at 37°C | Zonocerus variegatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
in potassium phosphate buffer (10 mM), Tris/HCl buffer (10 mM), and in glycine/NaOH buffer (100 mM) | Zonocerus variegatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Zonocerus variegatus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.155 | - |
phalaenopsine | in 100 mM glycine/NaOH buffer pH 9.0, at 37°C | Zonocerus variegatus | |
0.454 | - |
heliotrine | in 100 mM glycine/NaOH buffer pH 9.0, at 37°C | Zonocerus variegatus | |
37.64 | - |
atropine | in 100 mM glycine/NaOH buffer pH 9.0, at 37°C | Zonocerus variegatus | |
80.5 | - |
monocrotaline | in 100 mM glycine/NaOH buffer pH 9.0, at 37°C | Zonocerus variegatus | |
916.7 | - |
senecionine | in 100 mM glycine/NaOH buffer pH 9.0, at 37°C | Zonocerus variegatus |