Cloned (Comment) | Organism |
---|---|
several gene constructs are expressed in Escherichia coli DH5alpha, S17-1, S17-lambdapir and HMS174(DE3) | Burkholderia sp. |
Protein Variants | Comment | Organism |
---|---|---|
additional information | disruptants of Burkholderia sp. NK8 are generated by omega cassette interposon mutagenesis: NDBA1, NCAD, NCRD and NBALZ. Mutagenesis shows the involvement of the cbeABCD genes in the oxidation of 2-, 3-, 4-chlorobenzoate and benzoate in NK8 | Burkholderia sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | - |
Burkholderia sp. | |
Sulfide | - |
Burkholderia sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-chlorobenzoate + NADH + H+ + O2 | Burkholderia sp. | - |
catechol + chloride + NAD+ + CO2 | - |
? | |
2-chlorobenzoate + NADH + H+ + O2 | Burkholderia sp. NK8 | - |
catechol + chloride + NAD+ + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia sp. | - |
isolated from soil | - |
Burkholderia sp. NK8 | - |
isolated from soil | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2-chlorobenzoate + NADH + H+ + O2 = catechol + chloride + NAD+ + CO2 | broad substrate specificity, ability to transform all 3 monochlorobenzoates almost equally well. Enzyme appears to lack absolute regioselectivity | Burkholderia sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0013 | - |
cells are grown on 3-chlorobenzoate, benzoate as substrate for assay | Burkholderia sp. |
0.0024 | - |
recombinant cells are grown on LB/IPTG, benzoate as substrate for assay | Burkholderia sp. |
0.0032 | - |
cells are grown on benzoate, benzoate as substrate for assay | Burkholderia sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-chlorobenzoate + NADH + H+ + O2 | - |
Burkholderia sp. | catechol + chloride + NAD+ + CO2 | - |
? | |
2-chlorobenzoate + NADH + H+ + O2 | no growth on 2-chlorobenzoate | Burkholderia sp. | catechol + chloride + NAD+ + CO2 | - |
? | |
2-chlorobenzoate + NADH + H+ + O2 | - |
Burkholderia sp. NK8 | catechol + chloride + NAD+ + CO2 | - |
? | |
2-chlorobenzoate + NADH + H+ + O2 | no growth on 2-chlorobenzoate | Burkholderia sp. NK8 | catechol + chloride + NAD+ + CO2 | - |
? | |
2-chlorobenzoate + NADH + H+ + O2 | - |
Burkholderia sp. | 3-chlorocatechol + NAD+ + CO2 | - |
? | |
2-chlorobenzoate + NADH + H+ + O2 | - |
Burkholderia sp. NK8 | 3-chlorocatechol + NAD+ + CO2 | - |
? | |
3-chlorobenzoate + NADH + H+ + O2 | - |
Burkholderia sp. | 4-chlorocatechol + NAD+ + CO2 | - |
? | |
3-chlorobenzoate + NADH + H+ + O2 | - |
Burkholderia sp. NK8 | 4-chlorocatechol + NAD+ + CO2 | - |
? | |
3-chlorobenzoate + NADH + H+ + O2 | - |
Burkholderia sp. | 3-chlorocatechol + NAD+ + CO2 | - |
? | |
4-chlorobenzoate + NADH + H+ + O2 | - |
Burkholderia sp. | 4-chlorocatechol + NAD+ + CO2 | - |
? | |
benzoate + NADH + H+ + O2 | 2-chloro-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid as unstable intermediate | Burkholderia sp. | catechol + NAD+ + CO2 | - |
? | |
benzoate + NADH + H+ + O2 | 2-chloro-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid as unstable intermediate | Burkholderia sp. NK8 | catechol + NAD+ + CO2 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Burkholderia sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | assay at | Burkholderia sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Burkholderia sp. | |
NADH | - |
Burkholderia sp. |