Cloned (Comment) | Organism |
---|---|
expression of mutant enzymes in Escherichia coli BL21-Codon Plus | Acinetobacter sp. |
Protein Variants | Comment | Organism |
---|---|---|
D218A | no activity | Acinetobacter sp. |
D218E | no activity | Acinetobacter sp. |
D218N | no activity | Acinetobacter sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
apha, beta, 3 * 52000 + 3 * 24000, SDS-PAGE, native mass by gel filtration | Acinetobacter sp. |
52000 | - |
apha, beta, 3 * 52000 + 3 * 24000, SDS-PAGE, native mass by gel filtration | Acinetobacter sp. |
220000 | - |
gel filtration, wild type and mutant enzymes | Acinetobacter sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
anthranilic acid + NAD(P)H + O2 + H2O | Acinetobacter sp. | initial step in aerobic degradation of aromatic compounds, enables growth on anthranilate as sole carbon source | catechol + CO2 + NAD(P)+ + NH3 | - |
? | |
anthranilic acid + NAD(P)H + O2 + H2O | Acinetobacter sp. ADP1 | initial step in aerobic degradation of aromatic compounds, enables growth on anthranilate as sole carbon source | catechol + CO2 + NAD(P)+ + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter sp. | - |
- |
- |
Acinetobacter sp. ADP1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild type and mutant enzymes | Acinetobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
anthranilic acid + NAD(P)H + O2 + H2O | initial step in aerobic degradation of aromatic compounds, enables growth on anthranilate as sole carbon source | Acinetobacter sp. | catechol + CO2 + NAD(P)+ + NH3 | - |
? | |
anthranilic acid + NAD(P)H + O2 + H2O | initial step in aerobic degradation of aromatic compounds, enables growth on anthranilate as sole carbon source | Acinetobacter sp. ADP1 | catechol + CO2 + NAD(P)+ + NH3 | - |
? | |
anthranilic acid + NADH + O2 + H2O | - |
Acinetobacter sp. | catechol + CO2 + NAD+ + NH3 | - |
? | |
anthranilic acid + NADH + O2 + H2O | - |
Acinetobacter sp. ADP1 | catechol + CO2 + NAD+ + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | apha, beta, 3 * 52000 + 3 * 24000, SDS-PAGE, native mass by gel filtration | Acinetobacter sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
oxygenase component completely inactive at pH 9 | Acinetobacter sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
iron-sulfur centre | proximal Rieske [2Fe-2S] center | Acinetobacter sp. | |
NADH | - |
Acinetobacter sp. | |
NADPH | - |
Acinetobacter sp. |