Cloned (Comment) | Organism |
---|---|
expression from HeLa cell lines and from transient HEK293 cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, crystal structures of full-length human LH3 in complex with cofactors and donor substrates provide a molecular understanding of the biochemical knowledge underlying the multiple functions of this enzyme | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[procollagen]-L-lysine + 2-oxoglutarate + O2 | Homo sapiens | - |
[procollagen]-(2S,5R)-5-hydroxy-L-lysine + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O60568 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
[procollagen]-L-lysine + 2-oxoglutarate + O2 | - |
Homo sapiens | [procollagen]-(2S,5R)-5-hydroxy-L-lysine + succinate + CO2 | - |
? | |
[procollagen]-L-lysine + 2-oxoglutarate + O2 | human lysyl hydroxylase 3 (LH3/PLOD3) is a multifunctional collagen lysyl hydroxylase and glycosyltransferase LH3. Two distinct catalytic sites at the N- and C-terminal boundaries of each monomer are separated by an accessory domain. Collagen glucosyltransferase (EC 2.4.1.66) and procollagen galactosyltransferase (EC 2.4.1.50) activities localize at the N-terminus of the enzyme, whereas the lysyl hydroxylase activity (EC 1.14.11.4) is segregated at the LH3 C-terminus | Homo sapiens | [procollagen]-(2S,5R)-5-hydroxy-L-lysine + succinate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | two distinct catalytic sites at the N- and C-terminal boundaries of each monomer are separated by an accessory domain. Dimerization is essential for lysyl hydroxylase activity, whereas disruption of physiological dimers does not significantly perturb the N-terminal glycosyltransferase activities of LH3 | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
LH3/PLOD3 | - |
Homo sapiens |
lysyl hydroxylase 3 | - |
Homo sapiens |
multifunctional collagen lysyl hydroxylase | - |
Homo sapiens |