Literature summary for 1.13.11.55 extracted from

Zhang, H.; Guo, W.; Xu, C.; Zhou, H.; Chen, X.
Site-specific mutagenesis and functional analysis of active sites of sulfur oxygenase reductase from Gram-positive moderate thermophile Sulfobacillus acidophilus TPY (2013), Microbiol. Res., 168, 654-660.

Search for more literature for 1.13.11.55

Activating Compound

Activating Compound	Comment	Organism	Structure
glutathione	required for activity	Sulfobacillus acidophilus

Cloned(Commentary)

Cloned (Comment)	Organism
gene sor, DNA and amino acid sequence determination, comparison, and analysis, phylogenetic tree, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain B21 in inclusion bodies	Sulfobacillus acidophilus

Protein Variants

Protein Variants	Comment	Organism
C101S	site-directed mutagenesis, 10% reamining activity compared to the wild-type enzyme	Sulfobacillus acidophilus
C104S	site-directed mutagenesis, 47% reamining activity compared to the wild-type enzyme	Sulfobacillus acidophilus
C31S	site-directed mutagenesis, inactive mutant	Sulfobacillus acidophilus
E114A	site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme	Sulfobacillus acidophilus
H86A	site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme	Sulfobacillus acidophilus
H90A	site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme	Sulfobacillus acidophilus
additional information	mutations of putative SOR active site residues, C31, C101, C104, H86 and H90, and E114: replacement of any cysteine residues reduced SORactivity by 53-100%, while the mutants of H86A, H90A and E114A lost their enzyme activities largely, only remaining 20%, 19% and 32% activity of the wild type SOR respectively	Sulfobacillus acidophilus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
35000	-	x * 35000, SDS-PAGE	Sulfobacillus acidophilus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
4 sulfur + 4 H2O + O2	Sulfobacillus acidophilus	-	2 hydrogen sulfide + 2 bisulfite + 2 H+	-	?
4 sulfur + 4 H2O + O2	Sulfobacillus acidophilus TPY	-	2 hydrogen sulfide + 2 bisulfite + 2 H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Sulfobacillus acidophilus	-	isolated from a hydrothermal vent in the Pacific Ocean, gene sor	-
Sulfobacillus acidophilus TPY	-	isolated from a hydrothermal vent in the Pacific Ocean, gene sor	-

Purification (Commentary)

Purification (Comment)	Organism
solubilized recombinant wild-type and mutant enzymes from Escherichia coli strain B21 inclusion bodies by nickel affinity chromatography, and refolded by a four-step dialysis	Sulfobacillus acidophilus

Renatured (Commentary)

Renatured (Comment)	Organism
recombinant wild-type and mutant enzymes from Escherichia coli strain B21 inclusion bodies, purified by nickel affinity chromatography, are refolded by a four-step dialysis using 20 mM Tris/HCl, 150 mM NaCl, pH 8.14, containing 2 mM reduced glutathione, 0.02 mM oxidized glutathione, 5% glycerol, 0.005% Tween 20, and 0.1 mM ferric citrate with 6 M urea in the first step, 3 M urea in the second step, and 1 M urea in the third step	Sulfobacillus acidophilus

Renatured (Comment)

Organism

recombinant wild-type and mutant enzymes from Escherichia coli strain B21 inclusion bodies, purified by nickel affinity chromatography, are refolded by a four-step dialysis using 20 mM Tris/HCl, 150 mM NaCl, pH 8.14, containing 2 mM reduced glutathione, 0.02 mM oxidized glutathione, 5% glycerol, 0.005% Tween 20, and 0.1 mM ferric citrate with 6 M urea in the first step, 3 M urea in the second step, and 1 M urea in the third step

Sulfobacillus acidophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.21	-	wild-type enzyme in absence of GSH, pH 8.0, 80°C	Sulfobacillus acidophilus
1.75	-	mutant C101S, pH 8.0, 80°C	Sulfobacillus acidophilus
3.28	-	mutant H90A, pH 8.0, 80°C	Sulfobacillus acidophilus
3.47	-	mutant H86A, pH 8.0, 80°C	Sulfobacillus acidophilus
5.46	-	mutant E114A, pH 8.0, 80°C	Sulfobacillus acidophilus
8.09	-	mutant C104S, pH 8.0, 80°C	Sulfobacillus acidophilus
17.17	-	wild-type enzyme in presence of GSH, pH 8.0, 80°C	Sulfobacillus acidophilus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4 sulfur + 4 H2O + O2	-	Sulfobacillus acidophilus	2 hydrogen sulfide + 2 bisulfite + 2 H+	-	?
4 sulfur + 4 H2O + O2	-	Sulfobacillus acidophilus TPY	2 hydrogen sulfide + 2 bisulfite + 2 H+	-	?

Subunits

Subunits	Comment	Organism
?	x * 35000, SDS-PAGE	Sulfobacillus acidophilus

Synonyms

Synonyms	Comment	Organism
SOR	-	Sulfobacillus acidophilus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
80	-	assay at	Sulfobacillus acidophilus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Sulfobacillus acidophilus