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Literature summary for 1.13.11.18 extracted from
- The initial product and properties of the sulfur-oxidizing enzyme of thiobacilli (1966), Biochim. Biophys. Acta, 122, 22-33.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | similar stimulating effect as 2,2' dipyridyl | Thiobacillus thioparus |  |
| 2,2'-dipyridyl | stimulation at low concentrations e.g. 0.1 mM, may protect glutathione from destruction | Thiobacillus thioparus | |
| catalase | - |
Thiobacillus thioparus |
General Stability
| General Stability | Organism |
|---|---|
| 2,2'-dipyridyl stabilizes cofactor glutathione, activity is lost upon freezing and thawing | Thiobacillus thioparus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 0.1 mM | Thiobacillus thioparus | |
| Cu2+ | 0.1 mM | Thiobacillus thioparus | |
| diethyldithiocarbamate | - |
Thiobacillus thioparus | |
| EDTA | - |
Thiobacillus thioparus | |
| FAD | - |
Thiobacillus thioparus | |
| Fe2+ | - |
Thiobacillus thioparus | |
| Fe3+ | - |
Thiobacillus thioparus | |
| FMN | - |
Thiobacillus thioparus | |
| H2O2 | strong inhibition, reversible by subsequent addition of catalase and glutathione | Thiobacillus thioparus | |
| Zn2+ | 0.1 mM | Thiobacillus thioparus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6.3 | - |
glutathione | - |
Thiobacillus thioparus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| non-heme iron | - |
Acidithiobacillus thiooxidans | |
| non-heme iron | 0.000087 mmol of iron per mg of enzyme | Thiobacillus thioparus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sulfur + O2 + H2O | Thiobacillus thioparus | - |
sulfite | - |
ir | |
| sulfur + O2 + H2O | Acidithiobacillus thiooxidans | - |
sulfite | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acidithiobacillus thiooxidans | - |
- |
- |
| Thiobacillus thioparus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 30% ethanol, DEAE-cellulose | Thiobacillus thioparus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.25 | - |
- |
Thiobacillus thioparus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, DEAE-cellulose fraction, 7 d, almost complete loss of activity | Thiobacillus thioparus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione + sulfite + 2 H+ | - |
Thiobacillus thioparus | S-sulfanylglutathione + O2 + H2O | - |
r | |
| sulfur + O2 + H2O | - |
Thiobacillus thioparus | sulfite | - |
ir | |
| sulfur + O2 + H2O | - |
Acidithiobacillus thiooxidans | sulfite | - |
ir | |
| sulfur + O2 + H2O | - |
Thiobacillus thioparus | sulfite | S2O32- may be formed through a secondary, non-enzymatic reaction | ir | |
| sulfur + O2 + H2O | - |
Acidithiobacillus thiooxidans | sulfite | S2O32- may be formed through a secondary, non-enzymatic reaction | ir | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| glutathione | - |
Thiobacillus thioparus | |
| glutathione | - |
Acidithiobacillus thiooxidans | |
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