Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta cells | Irpex lacteus |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 0.2 M ammonium acetate, 0.1 M sodium acetate trihydrate and 34% (w/v) PEG 4000 | Irpex lacteus |
Protein Variants | Comment | Organism |
---|---|---|
W109F | the mutant shows decreased oxidative activity and decolorizing ability compared to the wild type enzyme | Irpex lacteus |
W147F | the mutant shows decreased oxidative activity and increased decolorizing ability compared to the wild type enzyme | Irpex lacteus |
W212F | the mutant shows decreased oxidative activity and increased decolorizing ability compared to the wild type enzyme | Irpex lacteus |
W264D | inactive | Irpex lacteus |
W264E | inactive | Irpex lacteus |
W264F | the mutant shows decreased oxidative activity and increased decolorizing ability compared to the wild type enzyme | Irpex lacteus |
W264G | inactive | Irpex lacteus |
W264H | the mutant shows decreased oxidative activity and decolorizing ability compared to the wild type enzyme | Irpex lacteus |
W264L | inactive | Irpex lacteus |
W264R | inactive | Irpex lacteus |
W264Y | the mutant shows decreased oxidative activity and decolorizing ability compared to the wild type enzyme | Irpex lacteus |
W380F | the mutant shows severely decreased oxidative activity and decolorizing ability compared to the wild type enzyme | Irpex lacteus |
W380G | the mutant shows decreased oxidative activity and decolorizing ability compared to the wild type enzyme | Irpex lacteus |
W380Y | the mutation does not weaken the first step of the catalytic cycles (oxidation of the resting state Fe3+ by H2O2) significantly, but it weakens the oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and other reducing substrates which has a larger size than H2O2 | Irpex lacteus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00898 | - |
2,6-dimethoxyphenol | wild type enzyme, at pH 3.5 and 35°C | Irpex lacteus | |
0.01947 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme W264R, at pH 3.5 and 35°C | Irpex lacteus | |
0.02008 | - |
H2O2 | mutant enzyme W380Y, at pH 3.5 and 35°C | Irpex lacteus | |
0.0276 | - |
Reactive Blue 19 | mutant enzyme W264F, at pH 3.5 and 35°C | Irpex lacteus | |
0.04264 | - |
2,6-dimethoxyphenol | mutant enzyme W264H, at pH 3.5 and 35°C | Irpex lacteus | |
0.0623 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme W264Y, at pH 3.5 and 35°C | Irpex lacteus | |
0.06321 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme W147F, at pH 3.5 and 35°C | Irpex lacteus | |
0.06644 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme W109F, at pH 3.5 and 35°C | Irpex lacteus | |
0.0665 | - |
Reactive Blue 19 | mutant enzyme W264H, at pH 3.5 and 35°C | Irpex lacteus | |
0.06729 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | wild type enzyme, at pH 3.5 and 35°C | Irpex lacteus | |
0.0689 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme W264H, at pH 3.5 and 35°C | Irpex lacteus | |
0.06919 | - |
2,6-dimethoxyphenol | mutant enzyme W147F, at pH 3.5 and 35°C | Irpex lacteus | |
0.07148 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme W212F, at pH 3.5 and 35°C | Irpex lacteus | |
0.07187 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme W264F, at pH 3.5 and 35°C | Irpex lacteus | |
0.07404 | - |
Reactive Blue 19 | mutant enzyme W212F, at pH 3.5 and 35°C | Irpex lacteus | |
0.08448 | - |
H2O2 | wild type enzyme, at pH 3.5 and 35°C | Irpex lacteus | |
0.08667 | - |
2,6-dimethoxyphenol | mutant enzyme W212F, at pH 3.5 and 35°C | Irpex lacteus | |
0.08688 | - |
Reactive Blue 19 | mutant enzyme W109F, at pH 3.5 and 35°C | Irpex lacteus | |
0.09759 | - |
Reactive Blue 19 | mutant enzyme W147F, at pH 3.5 and 35°C | Irpex lacteus | |
0.13987 | - |
Reactive Blue 19 | wild type enzyme, at pH 3.5 and 35°C | Irpex lacteus | |
0.17271 | - |
2,6-dimethoxyphenol | mutant enzyme W109F, at pH 3.5 and 35°C | Irpex lacteus | |
0.22905 | - |
2,6-dimethoxyphenol | mutant enzyme W264F, at pH 3.5 and 35°C | Irpex lacteus | |
0.27507 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme W380Y, at pH 3.5 and 35°C | Irpex lacteus | |
0.752 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme W380F, at pH 3.5 and 35°C | Irpex lacteus | |
0.953 | - |
2,6-dimethoxyphenol | mutant enzyme W264Y, at pH 3.5 and 35°C | Irpex lacteus | |
1.082 | - |
2,6-dimethoxyphenol | mutant enzyme W264R, at pH 3.5 and 35°C | Irpex lacteus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Irpex lacteus | A0A2P1C6N4 | - |
- |
Irpex lacteus F17 | A0A2P1C6N4 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Superdex 75 gel filtration | Irpex lacteus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+ | - |
Irpex lacteus | oxidized 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O | - |
? | |
2,6-dimethoxyphenol + H2O2 + H+ | - |
Irpex lacteus | oxidized 2,6-dimethoxyphenol + H2O | - |
? | |
2,6-dimethoxyphenol + H2O2 + H+ | - |
Irpex lacteus F17 | oxidized 2,6-dimethoxyphenol + H2O | - |
? | |
Direct Sky Blue 5B + H2O2 + H+ | - |
Irpex lacteus | oxidized Direct Sky Blue 5B + H2O | - |
? | |
guaiacol + H2O2 + H+ | - |
Irpex lacteus | oxidized guaiacol + H2O | - |
? | |
guaiacol + H2O2 + H+ | - |
Irpex lacteus F17 | oxidized guaiacol + H2O | - |
? | |
Methyl Orange + H2O2 + H+ | - |
Irpex lacteus | oxidized Methyl Orange + H2O | - |
? | |
Reactive Blue 19 + H2O2 + H+ | - |
Irpex lacteus | oxidized Reactive Blue 19 + H2O | - |
? | |
Reactive Blue 19 + H2O2 + H+ | - |
Irpex lacteus F17 | oxidized Reactive Blue 19 + H2O | - |
? | |
Reactive Blue 4 + H2O2 + H+ | - |
Irpex lacteus | oxidized Reactive Blue 4 + H2O | - |
? | |
Reactive Blue 4 + H2O2 + H+ | - |
Irpex lacteus F17 | oxidized Reactive Blue 4 + H2O | - |
? | |
Reactive Blue 5 + H2O2 + H+ | - |
Irpex lacteus | oxidized Reactive Blue 5 + H2O | - |
? | |
Reactive Blue 5 + H2O2 + H+ | - |
Irpex lacteus F17 | oxidized Reactive Blue 5 + H2O | - |
? | |
Reactive Violet 5 + H2O2 + H+ | - |
Irpex lacteus | oxidized Reactive Violet 5 + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
dye-decolorizing peroxidase | - |
Irpex lacteus |
DyP4 | - |
Irpex lacteus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
18.28 | - |
2,6-dimethoxyphenol | mutant enzyme W264R, at pH 3.5 and 35°C | Irpex lacteus | |
46.11 | - |
Reactive Blue 19 | mutant enzyme W264F, at pH 3.5 and 35°C | Irpex lacteus | |
52.78 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme W264R, at pH 3.5 and 35°C | Irpex lacteus | |
54.29 | - |
2,6-dimethoxyphenol | mutant enzyme W264H, at pH 3.5 and 35°C | Irpex lacteus | |
56.25 | - |
2,6-dimethoxyphenol | mutant enzyme W264Y, at pH 3.5 and 35°C | Irpex lacteus | |
58.99 | - |
2,6-dimethoxyphenol | wild type enzyme, at pH 3.5 and 35°C | Irpex lacteus | |
63.75 | - |
2,6-dimethoxyphenol | mutant enzyme W212F, at pH 3.5 and 35°C | Irpex lacteus | |
69.65 | - |
2,6-dimethoxyphenol | mutant enzyme W109F, at pH 3.5 and 35°C | Irpex lacteus | |
71.21 | - |
2,6-dimethoxyphenol | mutant enzyme W264F, at pH 3.5 and 35°C | Irpex lacteus | |
72.66 | - |
2,6-dimethoxyphenol | mutant enzyme W147F, at pH 3.5 and 35°C | Irpex lacteus | |
86.87 | - |
Reactive Blue 19 | mutant enzyme W264H, at pH 3.5 and 35°C | Irpex lacteus | |
88.38 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme W380Y, at pH 3.5 and 35°C | Irpex lacteus | |
108.79 | - |
Reactive Blue 19 | mutant enzyme W212F, at pH 3.5 and 35°C | Irpex lacteus | |
112.63 | - |
Reactive Blue 19 | mutant enzyme W109F, at pH 3.5 and 35°C | Irpex lacteus | |
148.99 | - |
Reactive Blue 19 | mutant enzyme W147F, at pH 3.5 and 35°C | Irpex lacteus | |
165.65 | - |
H2O2 | mutant enzyme W380Y, at pH 3.5 and 35°C | Irpex lacteus | |
187.9 | - |
Reactive Blue 19 | wild type enzyme, at pH 3.5 and 35°C | Irpex lacteus | |
243.13 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme W264Y, at pH 3.5 and 35°C | Irpex lacteus | |
316.16 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme W380F, at pH 3.5 and 35°C | Irpex lacteus | |
384.85 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme W264F, at pH 3.5 and 35°C | Irpex lacteus | |
602.5 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme W109F, at pH 3.5 and 35°C | Irpex lacteus | |
665.1 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme W212F, at pH 3.5 and 35°C | Irpex lacteus | |
684.34 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme W264H, at pH 3.5 and 35°C | Irpex lacteus | |
786.7 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme W147F, at pH 3.5 and 35°C | Irpex lacteus | |
790 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | wild type enzyme, at pH 3.5 and 35°C | Irpex lacteus | |
1025 | - |
H2O2 | wild type enzyme, at pH 3.5 and 35°C | Irpex lacteus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Irpex lacteus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
16.9 | - |
2,6-dimethoxyphenol | mutant enzyme W264R, at pH 3.5 and 35°C | Irpex lacteus | |
59 | - |
2,6-dimethoxyphenol | mutant enzyme W264Y, at pH 3.5 and 35°C | Irpex lacteus | |
311 | - |
2,6-dimethoxyphenol | mutant enzyme W264F, at pH 3.5 and 35°C | Irpex lacteus | |
321 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme W380Y, at pH 3.5 and 35°C | Irpex lacteus | |
403 | - |
2,6-dimethoxyphenol | mutant enzyme W109F, at pH 3.5 and 35°C | Irpex lacteus | |
420 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme W380F, at pH 3.5 and 35°C | Irpex lacteus | |
730 | - |
2,6-dimethoxyphenol | mutant enzyme W212F, at pH 3.5 and 35°C | Irpex lacteus | |
1050 | - |
2,6-dimethoxyphenol | mutant enzyme W147F, at pH 3.5 and 35°C | Irpex lacteus | |
1270 | - |
2,6-dimethoxyphenol | mutant enzyme W264H, at pH 3.5 and 35°C | Irpex lacteus | |
1300 | - |
Reactive Blue 19 | mutant enzyme W109F, at pH 3.5 and 35°C | Irpex lacteus | |
1310 | - |
Reactive Blue 19 | mutant enzyme W264H, at pH 3.5 and 35°C | Irpex lacteus | |
1340 | - |
Reactive Blue 19 | wild type enzyme, at pH 3.5 and 35°C | Irpex lacteus | |
1470 | - |
Reactive Blue 19 | mutant enzyme W212F, at pH 3.5 and 35°C | Irpex lacteus | |
1530 | - |
Reactive Blue 19 | mutant enzyme W147F, at pH 3.5 and 35°C | Irpex lacteus | |
1670 | - |
Reactive Blue 19 | mutant enzyme W264F, at pH 3.5 and 35°C | Irpex lacteus | |
2710 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme W264R, at pH 3.5 and 35°C | Irpex lacteus | |
3900 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme W264Y, at pH 3.5 and 35°C | Irpex lacteus | |
5350 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme W264F, at pH 3.5 and 35°C | Irpex lacteus | |
6570 | - |
2,6-dimethoxyphenol | wild type enzyme, at pH 3.5 and 35°C | Irpex lacteus | |
8250 | - |
H2O2 | mutant enzyme W380Y, at pH 3.5 and 35°C | Irpex lacteus | |
9030 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme W109F, at pH 3.5 and 35°C | Irpex lacteus | |
9300 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme W212F, at pH 3.5 and 35°C | Irpex lacteus | |
9930 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme W264H, at pH 3.5 and 35°C | Irpex lacteus | |
11700 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | wild type enzyme, at pH 3.5 and 35°C | Irpex lacteus | |
12100 | - |
H2O2 | wild type enzyme, at pH 3.5 and 35°C | Irpex lacteus | |
12500 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme W147F, at pH 3.5 and 35°C | Irpex lacteus |