Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Rhodococcus jostii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | 1 mM, 5.4fold activation in assay with lignin. Presence of Mn2+ is required, Km value is 8.4 mM, and data are not consistent with DypB acting as a Mn peroxidase. Breakdown of wheat straw lignocellulose by recombinant enzyme is observed over 24-48 h in the presence of 1 mM MnCl2 | Rhodococcus jostii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rhodococcus jostii | enzyme DypB has a significant role in lignin degradation in Rhodococcus jostii RHA1, is able to oxidize both polymeric lignin and a lignin model compound, and appears to have both Mn(II) and lignin oxidation sites | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus jostii | Q0SE24 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-(4-hydroxy-3-methoxyphenyl)-2-(2-methoxyphenoxy)propane-1,3-diol + H2O2 | - |
Rhodococcus jostii | vanillin + hydroxyacetaldehyde + guaiacol | Calpha-Cbeta bond cleavage of substrate takes place. This reaction is inhibited by addition of diaphorase, consistent with a radical mechanism for C-C bond cleavage | ? | |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2 | - |
Rhodococcus jostii | ? + H2O | - |
? | |
lignin + H2O2 | substrate is Kraft lignin | Rhodococcus jostii | ? + H2O | - |
? | |
lignocellulose + H2O2 | substrate is wheat straw lignocellulose | Rhodococcus jostii | ? + H2O | - |
? | |
additional information | enzyme DypB has a significant role in lignin degradation in Rhodococcus jostii RHA1, is able to oxidize both polymeric lignin and a lignin model compound, and appears to have both Mn(II) and lignin oxidation sites | Rhodococcus jostii | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DypB | - |
Rhodococcus jostii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.6 | - |
substrate 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) | Rhodococcus jostii |
General Information | Comment | Organism |
---|---|---|
physiological function | wild-type Rhodococcus jostii RHA1 shows hydrogen peroxide-dependent activity, whereas the DypB knockout strain shows no observable activity in the presence or absence of hydrogen peroxide and a significant decrease in activity compared to that of the wild-type strain | Rhodococcus jostii |