Activating Compound | Comment | Organism | Structure |
---|---|---|---|
SDS | enhances the PPO activity in fruits, with pulp showing a stronger increase than skin | Mangifera indica |
Application | Comment | Organism |
---|---|---|
food industry | inhibition of catechol oxidase diminishes browning in Ataulfo mango and elevates food quality | Mangifera indica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | complete inhibition at 0.02 mM | Mangifera indica | |
ascorbate | complete inhibition at 2 mM, 94% inhibition at 0.2 mM | Mangifera indica | |
benzoic acid | 56% inhibition at 20 mM, no inhibition at 0.02-2.0 mM | Mangifera indica | |
Citric acid | complete inhibition at 20 mM, 47% inhibition at 2 mM | Mangifera indica | |
EDTA | 73% inhibition at 2 mM, 31% inhibition at 0.2 mM | Mangifera indica | |
glutathione | complete inhibition at 2 mM, 94% inhibition at 0.2 mM | Mangifera indica | |
kojic acid | 96% inhibition at 2 mM, 60% inhibition at 0.2 mM | Mangifera indica | |
meta-bisulfite | complete inhibition at 0.2 mM, 76% inhibition at 0.02 mM | Mangifera indica | |
NaCl | 54% inhibition at 2 mM, 19% inhibition at 0.2 mM | Mangifera indica | |
Sodium azide | complete inhibition at 2 mM, 99% inhibition at 0.2 mM | Mangifera indica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics at different pH and in different buffer systems, overview | Mangifera indica | |
0.41 | - |
catechol | pH 6.2, 25°C, sodium phosphate buffer | Mangifera indica | |
0.68 | - |
catechol | pH 6.0, 25°C, MES buffer | Mangifera indica | |
0.79 | - |
pyrogallol | pH 6.0, 25°C, MES buffer | Mangifera indica | |
0.83 | - |
pyrogallol | pH 5.8, 25°C, sodium phosphate buffer | Mangifera indica | |
0.87 | - |
catechol | pH 5.8, 25°C, sodium phosphate buffer | Mangifera indica | |
1.25 | - |
catechol | pH 5.8, 25°C, MES buffer | Mangifera indica | |
1.26 | - |
pyrogallol | pH 5.4, 25°C, MES buffer | Mangifera indica | |
1.31 | - |
3-methylcatechol | pH 5.6, 25°C, MES buffer | Mangifera indica | |
3.69 | - |
3-methylcatechol | pH 5.4, 25°C, sodium acetate buffer | Mangifera indica | |
4.6 | - |
gallate | pH 5.8, 25°C | Mangifera indica | |
7.31 | - |
pyrogallol | pH 5.6, 25°C, sodium acetate buffer | Mangifera indica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | a copper-containing enzyme | Mangifera indica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mangifera indica | D2XZ13 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | PPO activity is higher in the skin compared to the pulp and increases with ripeness | Mangifera indica | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 3-methylcatechol + O2 | - |
Mangifera indica | 2 3-methyl-1,2-benzoquinone + 2 H2O | - |
? | |
2 catechol + O2 | - |
Mangifera indica | 2 1,2-benzoquinone + 2 H2O | - |
? | |
gallate + O2 | - |
Mangifera indica | ? | - |
? | |
additional information | substrate specificity, overview | Mangifera indica | ? | - |
? | |
protocatechuate + O2 | - |
Mangifera indica | ? | - |
? | |
pyrogallol + O2 | - |
Mangifera indica | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 144000 + x * 112000 + x * 72000 + x * 23000, SDS-PAGE, differently distributed in fruit pulp and skin, some might by degeneration products | Mangifera indica |
Synonyms | Comment | Organism |
---|---|---|
polyphenol oxidase | - |
Mangifera indica |
PPO | - |
Mangifera indica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Mangifera indica |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
30 min, 50% activity remaining | Mangifera indica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.4 | 6.4 | dependent on the substrate, overview | Mangifera indica |