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Literature summary for 1.1.99.28 extracted from

  • Aziz, M.; Yusof, Y.; Kulbe, K.
    Production and application of glucose-fructose oxidoreductase for conversion of pineapple juice sugars (2011), Afr. J. Microbiol. Res., 5, 5046-5052.
No PubMed abstract available

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
Zymomonas mobilis
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
4 * 40000, SDS-PAGE Zymomonas mobilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose + D-fructose + H2O Zymomonas mobilis pineapple juice sugars, the enzyme is efficient in converting juice sugars of fruits juices having higher natural pH D-glucono-1,5-lactone + D-sorbitol
-
?
D-glucose + D-fructose + H2O Zymomonas mobilis DSM 473 pineapple juice sugars, the enzyme is efficient in converting juice sugars of fruits juices having higher natural pH D-glucono-1,5-lactone + D-sorbitol
-
?

Organism

Organism UniProt Comment Textmining
Zymomonas mobilis
-
-
-
Zymomonas mobilis DSM 473
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Sepharose CL-4B column chromatography and Source S column chromatography Zymomonas mobilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.6
-
raw extract, at pH 6.3 and 25°C Zymomonas mobilis
72
-
purified enzyme, at pH 6.3 and 25°C Zymomonas mobilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose + D-fructose + H2O pineapple juice sugars, the enzyme is efficient in converting juice sugars of fruits juices having higher natural pH Zymomonas mobilis D-glucono-1,5-lactone + D-sorbitol
-
?
D-glucose + D-fructose + H2O pineapple juice sugars, the enzyme is efficient in converting juice sugars of fruits juices having higher natural pH Zymomonas mobilis DSM 473 D-glucono-1,5-lactone + D-sorbitol
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 40000, SDS-PAGE Zymomonas mobilis

Synonyms

Synonyms Comment Organism
GFOR
-
Zymomonas mobilis

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Zymomonas mobilis