Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli OrigamiB(DE3)pLysS | Halomonas elongata |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
choline + 2 acceptor + H2O | Halomonas elongata | four electron oxidation with betaine aldehyde as intermediate, provides glycine-betaine as compatible solute | glycine-betaine + 2 reduced acceptor + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Halomonas elongata | - |
halophil | - |
Purification (Comment) | Organism |
---|---|
partial | Halomonas elongata |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.8 | - |
purified enzyme, pH 7.0, 25°C | Halomonas elongata |
Storage Stability | Organism |
---|---|
-20°C, phosphate buffer, pH 7.0, 2 M sorbitol, 20% glycerol | Halomonas elongata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
betaine aldehyde + phenazine methosulfate | - |
Halomonas elongata | glycine-betaine + ? | - |
? | |
choline + 2 acceptor + H2O | four electron oxidation with betaine aldehyde as intermediate, provides glycine-betaine as compatible solute | Halomonas elongata | glycine-betaine + 2 reduced acceptor + H+ | - |
? | |
choline + 2 acceptor + H2O | four electron oxidation with betaine aldehyde as intermediate | Halomonas elongata | glycine-betaine + 2 reduced acceptor + H+ | - |
? | |
choline + acceptor + phenazine methosulfate | - |
Halomonas elongata | betaine aldehyde + ? | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | FAD and pyrroloquinoline quinone has no effect on activity | Halomonas elongata |