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Literature summary for 1.1.3.B5 extracted from

  • Habib, M.; Trajkovic, M.; Fraaije, M.W.
    The biocatalytic synthesis of syringaresinol from 2,6-dimethoxy-4-allylphenol in one-pot using a tailored oxidase/peroxidase system (2018), ACS Catal., 8, 5549-5552 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.3.B5

Application

Application Comment Organism
synthesis synthesis of syringaresinol in a one-pot conversion containing eugenol oxidase (EUGO) and horseradish peroxidase (HRP) using 2,6-dimethoxy-4-allylphenol as a substrate. The hydrogen peroxide generated from the reaction of EUGO with the substrate is utilized by the HRP to convert the formed sinapyl alcohol into syringaresinol. Mutant I427A together with HRP are capable of efficiently producing syringaresinol as a major product. After optimization and upscaling to a semipreparative scale (1 gr), syringaresinol is obtained in 81% yield Rhodococcus jostii

Protein Variants

Protein Variants Comment Organism
I427A mutant is significantly more efficient with 2,6-dimethoxy-4-allylphenol than wild-type Rhodococcus jostii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00046
-
 2,6-dimethoxy-4-allylphenol mutant I427A, pH not specified in the publication, temperature not specified in the publication Rhodococcus jostii
0.00077
-
 2,6-dimethoxy-4-allylphenol wild-type, pH not specified in the publication, temperature not specified in the publication Rhodococcus jostii

Organism

Organism UniProt Comment Textmining
Rhodococcus jostii Q0SBK1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,6-dimethoxy-4-allylphenol + O2 + H2O
-
 Rhodococcus jostii sinapyl alcohol + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
EUGO
-
 Rhodococcus jostii
RHA1_ro03282
-
 Rhodococcus jostii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.37
-
 2,6-dimethoxy-4-allylphenol wild-type, pH not specified in the publication, temperature not specified in the publication Rhodococcus jostii
0.72
-
 2,6-dimethoxy-4-allylphenol mutant I427A, pH not specified in the publication, temperature not specified in the publication Rhodococcus jostii