Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Coprinopsis cinerea |
Protein Variants | Comment | Organism |
---|---|---|
L416F | mutation decreases the activity with aromatic alcohols but maintains the activity with trans,trans-2,4-hexadien-1-ol | Coprinopsis cinerea |
L416W | mutation decreases the activity with aromatic alcohols but maintains the activity with trans,trans-2,4-hexadien-1-ol | Coprinopsis cinerea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | - |
benzyl alcohol | wild-type, pH 7, 25°C | Coprinopsis cinerea | |
0.004 | - |
3-anisyl alcohol | wild-type, pH 7, 25°C | Coprinopsis cinerea | |
0.0057 | - |
3-Hydroxybenzyl alcohol | wild-type, pH 7, 25°C | Coprinopsis cinerea | |
0.0059 | - |
benzyl alcohol | mutant L416W, pH 7, 25°C | Coprinopsis cinerea | |
0.0063 | - |
Vanillyl alcohol | wild-type, pH 7, 25°C | Coprinopsis cinerea | |
0.007 | - |
(2E,4E)-hexa-2,4-dien-1-ol | mutant L416W, pH 7, 25°C | Coprinopsis cinerea | |
0.0076 | - |
4-anisyl alcohol | mutant L416W, pH 7, 25°C | Coprinopsis cinerea | |
0.0079 | - |
benzyl alcohol | mutant L416F, pH 7, 25°C | Coprinopsis cinerea | |
0.012 | - |
4-anisyl alcohol | wild-type, pH 7, 25°C | Coprinopsis cinerea | |
0.0156 | - |
(2E,4E)-hexa-2,4-dien-1-ol | wild-type, pH 7, 25°C | Coprinopsis cinerea | |
0.0164 | - |
(2E,4E)-hexa-2,4-dien-1-ol | mutant L416F, pH 7, 25°C | Coprinopsis cinerea | |
0.019 | - |
4-anisyl alcohol | mutant L416F, pH 7, 25°C | Coprinopsis cinerea | |
0.031 | - |
3-anisyl alcohol | mutant L416W, pH 7, 25°C | Coprinopsis cinerea | |
0.034 | - |
3-anisyl alcohol | mutant L416F, pH 7, 25°C | Coprinopsis cinerea | |
0.042 | - |
isovanillyl alcohol | wild-type, pH 7, 25°C | Coprinopsis cinerea | |
0.0483 | - |
veratryl alcohol | wild-type, pH 7, 25°C | Coprinopsis cinerea | |
0.0563 | - |
4-Hydroxybenzyl alcohol | wild-type, pH 7, 25°C | Coprinopsis cinerea | |
0.154 | - |
isovanillyl alcohol | mutant L416W, pH 7, 25°C | Coprinopsis cinerea | |
0.163 | - |
4-Hydroxybenzyl alcohol | mutant L416F, pH 7, 25°C | Coprinopsis cinerea | |
0.279 | - |
3-Hydroxybenzyl alcohol | mutant L416W, pH 7, 25°C | Coprinopsis cinerea | |
0.301 | - |
3-Hydroxybenzyl alcohol | mutant L416F, pH 7, 25°C | Coprinopsis cinerea | |
0.318 | - |
isovanillyl alcohol | mutant L416F, pH 7, 25°C | Coprinopsis cinerea | |
0.419 | - |
Vanillyl alcohol | mutant L416W, pH 7, 25°C | Coprinopsis cinerea | |
0.424 | - |
veratryl alcohol | mutant L416F, pH 7, 25°C | Coprinopsis cinerea | |
0.466 | - |
4-Hydroxybenzyl alcohol | mutant L416W, pH 7, 25°C | Coprinopsis cinerea | |
0.686 | - |
veratryl alcohol | mutant L416W, pH 7, 25°C | Coprinopsis cinerea | |
0.98 | - |
Vanillyl alcohol | mutant L416F, pH 7, 25°C | Coprinopsis cinerea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Coprinopsis cinerea | A0A2Z5WP81 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the amino acid sequence contains four and seven possible sites of N-glycosylation and O-glycosylation, respectively | Coprinopsis cinerea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,4E)-hexa-2,4-dien-1-ol + O2 | 282% of the activity with benzyl alcohol | Coprinopsis cinerea | ? + H2O2 | - |
? | |
2,4-dimethoxybenzyl alcohol + O2 | 286% of the activity with benzyl alcohol | Coprinopsis cinerea | 2,4-dimethoxybenzyl aldehyde + H2O2 | - |
? | |
2-anisyl alcohol + O2 | 96% of the activity with benzyl alcohol | Coprinopsis cinerea | 2-anisyl aldehyde + H2O2 | - |
? | |
3-anisyl alcohol + O2 | 297% of the activity with benzyl alcohol | Coprinopsis cinerea | 3-anisyl aldehyde + H2O2 | - |
? | |
3-hydroxybenzyl alcohol + O2 | 221% of the activity with benzyl alcohol | Coprinopsis cinerea | 3-hydroxybenzyl aldehyde + H2O2 | - |
? | |
4-anisyl alcohol + O2 | 298% of the activity with benzyl alcohol | Coprinopsis cinerea | 4-anisyl aldehyde + H2O2 | - |
? | |
4-hydroxybenzyl alcohol + O2 | 207% of the activity with benzyl alcohol | Coprinopsis cinerea | 4-hydroxybenzyl aldehyde + H2O2 | - |
? | |
benzyl alcohol + O2 | - |
Coprinopsis cinerea | benzaldehyde + H2O2 | - |
? | |
benzyl alcohol + O2 | - |
Coprinopsis cinerea | benzyl aldehyde + H2O2 | - |
? | |
cinnamyl alcohol + O2 | 289% of the activity with benzyl alcohol | Coprinopsis cinerea | cinnamyl aldehyde + H2O2 | - |
? | |
isovanillyl alcohol + O2 | 73% of the activity with benzyl alcohol | Coprinopsis cinerea | isovanillyl aldehyde + H2O2 | - |
? | |
additional information | no activity with mono- and disaccharides. No electron acceptor: DCIP | Coprinopsis cinerea | ? | - |
- |
|
vanillyl alcohol + O2 | - |
Coprinopsis cinerea | vanillyl aldehyde + H2O2 | - |
? | |
veratryl alcohol + O2 | 318% of the activity with benzyl alcohol | Coprinopsis cinerea | veratryl aldehyde + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 62600, calculated from sequence, x * 65600, SDS-PAGE of endoH-treated protein | Coprinopsis cinerea |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.54 | - |
4-Hydroxybenzyl alcohol | mutant L416W, pH 7, 25°C | Coprinopsis cinerea | |
0.81 | - |
Vanillyl alcohol | mutant L416W, pH 7, 25°C | Coprinopsis cinerea | |
1.17 | - |
3-Hydroxybenzyl alcohol | mutant L416W, pH 7, 25°C | Coprinopsis cinerea | |
2.05 | - |
isovanillyl alcohol | mutant L416W, pH 7, 25°C | Coprinopsis cinerea | |
2.96 | - |
veratryl alcohol | mutant L416W, pH 7, 25°C | Coprinopsis cinerea | |
3.49 | - |
4-anisyl alcohol | mutant L416W, pH 7, 25°C | Coprinopsis cinerea | |
3.84 | - |
benzyl alcohol | mutant L416W, pH 7, 25°C | Coprinopsis cinerea | |
4.81 | - |
4-Hydroxybenzyl alcohol | wild-type, pH 7, 25°C | Coprinopsis cinerea | |
6.13 | - |
benzyl alcohol | wild-type, pH 7, 25°C | Coprinopsis cinerea | |
7.02 | - |
isovanillyl alcohol | wild-type, pH 7, 25°C | Coprinopsis cinerea | |
7.5 | - |
3-Hydroxybenzyl alcohol | mutant L416F, pH 7, 25°C | Coprinopsis cinerea | |
7.66 | - |
3-anisyl alcohol | wild-type, pH 7, 25°C | Coprinopsis cinerea | |
8.03 | - |
benzyl alcohol | mutant L416F, pH 7, 25°C | Coprinopsis cinerea | |
8.67 | - |
isovanillyl alcohol | mutant L416F, pH 7, 25°C | Coprinopsis cinerea | |
8.77 | - |
4-Hydroxybenzyl alcohol | mutant L416F, pH 7, 25°C | Coprinopsis cinerea | |
9.91 | - |
3-Hydroxybenzyl alcohol | wild-type, pH 7, 25°C | Coprinopsis cinerea | |
11.3 | - |
3-anisyl alcohol | mutant L416W, pH 7, 25°C | Coprinopsis cinerea | |
12.4 | - |
veratryl alcohol | mutant L416F, pH 7, 25°C | Coprinopsis cinerea | |
12.4 | - |
3-anisyl alcohol | mutant L416F, pH 7, 25°C | Coprinopsis cinerea | |
12.5 | - |
4-anisyl alcohol | wild-type, pH 7, 25°C | Coprinopsis cinerea | |
13.2 | - |
veratryl alcohol | wild-type, pH 7, 25°C | Coprinopsis cinerea | |
14.2 | - |
4-anisyl alcohol | mutant L416F, pH 7, 25°C | Coprinopsis cinerea | |
14.7 | - |
Vanillyl alcohol | wild-type, pH 7, 25°C | Coprinopsis cinerea | |
17.3 | - |
(2E,4E)-hexa-2,4-dien-1-ol | mutant L416W, pH 7, 25°C | Coprinopsis cinerea | |
21.3 | - |
Vanillyl alcohol | mutant L416F, pH 7, 25°C | Coprinopsis cinerea | |
48.3 | - |
(2E,4E)-hexa-2,4-dien-1-ol | wild-type, pH 7, 25°C | Coprinopsis cinerea | |
68.4 | - |
(2E,4E)-hexa-2,4-dien-1-ol | mutant L416F, pH 7, 25°C | Coprinopsis cinerea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Coprinopsis cinerea |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | - |
Coprinopsis cinerea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Coprinopsis cinerea |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Coprinopsis cinerea | calculated from sequence | - |
6.7 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
4-Hydroxybenzyl alcohol | mutant L416W, pH 7, 25°C | Coprinopsis cinerea | |
1.9 | - |
Vanillyl alcohol | mutant L416W, pH 7, 25°C | Coprinopsis cinerea | |
4.2 | - |
3-Hydroxybenzyl alcohol | mutant L416W, pH 7, 25°C | Coprinopsis cinerea | |
4.3 | - |
veratryl alcohol | mutant L416W, pH 7, 25°C | Coprinopsis cinerea | |
13.3 | - |
isovanillyl alcohol | mutant L416W, pH 7, 25°C | Coprinopsis cinerea | |
21.8 | - |
Vanillyl alcohol | mutant L416F, pH 7, 25°C | Coprinopsis cinerea | |
25 | - |
3-Hydroxybenzyl alcohol | mutant L416F, pH 7, 25°C | Coprinopsis cinerea | |
27.3 | - |
isovanillyl alcohol | mutant L416F, pH 7, 25°C | Coprinopsis cinerea | |
29.3 | - |
veratryl alcohol | mutant L416F, pH 7, 25°C | Coprinopsis cinerea | |
53.9 | - |
4-Hydroxybenzyl alcohol | mutant L416F, pH 7, 25°C | Coprinopsis cinerea | |
85.5 | - |
4-Hydroxybenzyl alcohol | wild-type, pH 7, 25°C | Coprinopsis cinerea | |
167 | - |
isovanillyl alcohol | wild-type, pH 7, 25°C | Coprinopsis cinerea | |
273 | - |
veratryl alcohol | wild-type, pH 7, 25°C | Coprinopsis cinerea | |
361 | - |
3-anisyl alcohol | mutant L416F, pH 7, 25°C | Coprinopsis cinerea | |
367 | - |
3-anisyl alcohol | mutant L416W, pH 7, 25°C | Coprinopsis cinerea | |
461 | - |
4-anisyl alcohol | mutant L416W, pH 7, 25°C | Coprinopsis cinerea | |
665 | - |
benzyl alcohol | mutant L416W, pH 7, 25°C | Coprinopsis cinerea | |
740 | - |
4-anisyl alcohol | mutant L416F, pH 7, 25°C | Coprinopsis cinerea | |
1020 | - |
benzyl alcohol | mutant L416F, pH 7, 25°C | Coprinopsis cinerea | |
1080 | - |
4-anisyl alcohol | wild-type, pH 7, 25°C | Coprinopsis cinerea | |
1750 | - |
3-Hydroxybenzyl alcohol | wild-type, pH 7, 25°C | Coprinopsis cinerea | |
1940 | - |
3-anisyl alcohol | wild-type, pH 7, 25°C | Coprinopsis cinerea | |
2350 | - |
Vanillyl alcohol | wild-type, pH 7, 25°C | Coprinopsis cinerea | |
2460 | - |
(2E,4E)-hexa-2,4-dien-1-ol | mutant L416W, pH 7, 25°C | Coprinopsis cinerea | |
3100 | - |
(2E,4E)-hexa-2,4-dien-1-ol | wild-type, pH 7, 25°C | Coprinopsis cinerea | |
4180 | - |
(2E,4E)-hexa-2,4-dien-1-ol | mutant L416F, pH 7, 25°C | Coprinopsis cinerea | |
5060 | - |
benzyl alcohol | wild-type, pH 7, 25°C | Coprinopsis cinerea |