Application | Comment | Organism |
---|---|---|
synthesis | expression variant carrying 4 mutations in the chimeric signal peptide (prealphaproK), plus mutations H91N/L170M in the mature protein, shows 350fold improved secretion (4.5 mg/l) in Saccharomyces cerevisiae and is stable. Expression in Pichia pastoris and fermentation in a fed-batch bioreactor enhances production to 25 mg/l. Both recombinant AAO from Saccharomyces cerevisiae and Pichia pastoris are subjected to the same N-terminal processing and have a similar pH activity profile, they differ in their kinetic parameters and thermostability | Pleurotus eryngii |
Protein Variants | Comment | Organism |
---|---|---|
H91N/L170M | expression variant carrying 4 mutations in the chimeric signal peptide (prealphaproK), plus mutations H91N/L170M in the mature protein, shows increased secretion upon expression in Pichia pastoris and Saccharomyces cerevisiae | Pleurotus eryngii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
4-methoxybenzyl alcohol | recombinant protein from glycosylation-deficient Saccharomyces cerevisiae, pH 6, 25°C | Pleurotus eryngii | |
0.023 | - |
4-methoxybenzyl alcohol | recombinant protein from wild-type Saccharomyces cerevisiae, pH 6, 25°C | Pleurotus eryngii | |
0.037 | - |
4-methoxybenzyl alcohol | recombinant protein from wild-type Pichia pastoris, pH 6, 25°C | Pleurotus eryngii | |
0.095 | - |
2,4-hexadien-1-ol | recombinant protein from glycosylation-deficient Saccharomyces cerevisiae, pH 6, 25°C | Pleurotus eryngii | |
0.096 | - |
2,4-hexadien-1-ol | recombinant protein from wild-type Saccharomyces cerevisiae, pH 6, 25°C | Pleurotus eryngii | |
0.106 | - |
2,4-hexadien-1-ol | recombinant protein from wild-type Pichia pastoris, pH 6, 25°C | Pleurotus eryngii | |
0.34 | - |
veratryl alcohol | recombinant protein from wild-type Saccharomyces cerevisiae, pH 6, 25°C | Pleurotus eryngii | |
0.36 | - |
veratryl alcohol | recombinant protein from glycosylation-deficient Saccharomyces cerevisiae, pH 6, 25°C | Pleurotus eryngii | |
0.37 | - |
benzyl alcohol | recombinant protein from wild-type Saccharomyces cerevisiae, pH 6, 25°C | Pleurotus eryngii | |
0.38 | - |
benzyl alcohol | recombinant protein from glycosylation-deficient Saccharomyces cerevisiae, pH 6, 25°C | Pleurotus eryngii | |
0.41 | - |
veratryl alcohol | recombinant protein from wild-type Pichia pastoris, pH 6, 25°C | Pleurotus eryngii | |
0.44 | - |
benzyl alcohol | recombinant protein from wild-type Pichia pastoris, pH 6, 25°C | Pleurotus eryngii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pleurotus eryngii | O94219 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | recombinant protein from wild-type Saccharomyces cerevisiae, 60% glycosylation | Pleurotus eryngii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-hexadien-1-ol + 2 O2 | - |
Pleurotus eryngii | 2,4-hexadienal + 2 H2O2 | - |
? | |
4-methoxybenzyl alcohol + O2 | - |
Pleurotus eryngii | 4-methoxybenzyl aldehyde + H2O2 | - |
? | |
benzyl alcohol + O2 | - |
Pleurotus eryngii | benzyl aldehyde + H2O2 | - |
? | |
veratryl alcohol + O2 | - |
Pleurotus eryngii | veratryl aldehyde + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 150000, recombinant protein from wild-type Saccharomyces cerevisiae, x * 63000, recombinant protein from glycosylation-deficient Saccharomyces cerevisiae, x * 63000, recombinant protein from wild-type Pichia pastoris | Pleurotus eryngii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
61.2 | - |
melting temperature, recombinant protein from glycosylation-deficient Saccharomyces cerevisiae | Pleurotus eryngii |
61.3 | - |
melting temperature, recombinant protein from wild-type Pichia pastoris | Pleurotus eryngii |
63 | - |
melting temperature, recombinant protein from wild-type Saccharomyces cerevisiae | Pleurotus eryngii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15.1 | - |
benzyl alcohol | recombinant protein from wild-type Saccharomyces cerevisiae, pH 6, 25°C | Pleurotus eryngii | |
23.4 | - |
benzyl alcohol | recombinant protein from glycosylation-deficient Saccharomyces cerevisiae, pH 6, 25°C | Pleurotus eryngii | |
24.6 | - |
veratryl alcohol | recombinant protein from wild-type Saccharomyces cerevisiae, pH 6, 25°C | Pleurotus eryngii | |
34.4 | - |
benzyl alcohol | recombinant protein from wild-type Pichia pastoris, pH 6, 25°C | Pleurotus eryngii | |
36.8 | - |
veratryl alcohol | recombinant protein from glycosylation-deficient Saccharomyces cerevisiae, pH 6, 25°C | Pleurotus eryngii | |
41 | - |
4-methoxybenzyl alcohol | recombinant protein from wild-type Saccharomyces cerevisiae, pH 6, 25°C | Pleurotus eryngii | |
47 | - |
2,4-hexadien-1-ol | recombinant protein from wild-type Saccharomyces cerevisiae, pH 6, 25°C | Pleurotus eryngii | |
56.9 | - |
4-methoxybenzyl alcohol | recombinant protein from glycosylation-deficient Saccharomyces cerevisiae, pH 6, 25°C | Pleurotus eryngii | |
56.9 | - |
veratryl alcohol | recombinant protein from wild-type Pichia pastoris, pH 6, 25°C | Pleurotus eryngii | |
62 | - |
2,4-hexadien-1-ol | recombinant protein from glycosylation-deficient Saccharomyces cerevisiae, pH 6, 25°C | Pleurotus eryngii | |
70 | - |
4-methoxybenzyl alcohol | recombinant protein from wild-type Pichia pastoris, pH 6, 25°C | Pleurotus eryngii | |
89 | - |
2,4-hexadien-1-ol | recombinant protein from wild-type Pichia pastoris, pH 6, 25°C | Pleurotus eryngii |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pleurotus eryngii | isoelelctric focusing, recombinant protein from wild-type Pichia pastoris | - |
4.5 |
Pleurotus eryngii | isoelectric focusing, recombinant protein from glycosylation-deficient Saccharomyces cerevisiae | - |
4.6 |
Pleurotus eryngii | isoelectric focusing, recombinant protein from wild-type Saccharomyces cerevisiae | - |
4.8 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
41 | - |
benzyl alcohol | recombinant protein from wild-type Saccharomyces cerevisiae, pH 6, 25°C | Pleurotus eryngii | |
62 | - |
benzyl alcohol | recombinant protein from glycosylation-deficient Saccharomyces cerevisiae, pH 6, 25°C | Pleurotus eryngii | |
72 | - |
veratryl alcohol | recombinant protein from wild-type Saccharomyces cerevisiae, pH 6, 25°C | Pleurotus eryngii | |
78 | - |
benzyl alcohol | recombinant protein from wild-type Pichia pastoris, pH 6, 25°C | Pleurotus eryngii | |
102 | - |
veratryl alcohol | recombinant protein from glycosylation-deficient Saccharomyces cerevisiae, pH 6, 25°C | Pleurotus eryngii | |
139 | - |
veratryl alcohol | recombinant protein from wild-type Pichia pastoris, pH 6, 25°C | Pleurotus eryngii | |
456 | - |
2,4-hexadien-1-ol | recombinant protein from wild-type Saccharomyces cerevisiae, pH 6, 25°C | Pleurotus eryngii | |
653 | - |
2,4-hexadien-1-ol | recombinant protein from glycosylation-deficient Saccharomyces cerevisiae, pH 6, 25°C | Pleurotus eryngii | |
840 | - |
2,4-hexadien-1-ol | recombinant protein from wild-type Pichia pastoris, pH 6, 25°C | Pleurotus eryngii | |
1782 | - |
4-methoxybenzyl alcohol | recombinant protein from wild-type Saccharomyces cerevisiae, pH 6, 25°C | Pleurotus eryngii | |
1909 | - |
4-methoxybenzyl alcohol | recombinant protein from wild-type Pichia pastoris, pH 6, 25°C | Pleurotus eryngii | |
2586 | - |
4-methoxybenzyl alcohol | recombinant protein from glycosylation-deficient Saccharomyces cerevisiae, pH 6, 25°C | Pleurotus eryngii |