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Literature summary for 1.1.3.7 extracted from
- Enzymatic versatility and thermostability of a new aryl-alcohol oxidase from Thermothelomyces thermophilus M77 (2020), Biochim. Biophys. Acta, 1864, 129681 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 1 mM, 119% of initial activity | Thermothelomyces thermophilus |  |
| Co2+ | 1 mM, 111% of initial activity | Thermothelomyces thermophilus | |
| K+ | 1 mM, 106% of initial activity | Thermothelomyces thermophilus | |
| Sr2+ | 1 mM, 106% of initial activity | Thermothelomyces thermophilus | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Aspergillus nidulans | Thermothelomyces thermophilus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| to 2.6 A resolution | Thermothelomyces thermophilus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.47 | - |
veratryl alcohol | pH 6, 30°C | Thermothelomyces thermophilus | |
| 0.49 | - |
cinnamyl alcohol | pH 6, 30°C | Thermothelomyces thermophilus | |
| 1.03 | - |
anisyl alcohol | pH 6, 30°C | Thermothelomyces thermophilus | |
| 13.02 | - |
5-hydroxymethylfurfural | pH 6, 30°C | Thermothelomyces thermophilus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | 1 mM, 62% of initial activity | Thermothelomyces thermophilus | |
| Cu2+ | 1 mM, 49% of initial activity | Thermothelomyces thermophilus | |
| ethanol | 20%, 93% of initial activity | Thermothelomyces thermophilus | |
| Fe2+ | 1 mM, 93% of initial activity | Thermothelomyces thermophilus | |
| Mg2+ | 1 mM, 91of initial activity | Thermothelomyces thermophilus | |
| SDS | 1%,81% of initial activity | Thermothelomyces thermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 79500 | - |
MALDI-TOF mass spectrometry | Thermothelomyces thermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermothelomyces thermophilus | G2PZJ2 | - |
- |
| Thermothelomyces thermophilus DSM 1799 | G2PZJ2 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | enzyme shows about 14% glycosylation | Thermothelomyces thermophilus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein | Thermothelomyces thermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-hydroxymethylfurfural + O2 | - |
Thermothelomyces thermophilus | 2,5-diformylfuran + 5-formylfuran-2-carboxylic acid + H2O2 | - |
? | |
| 5-hydroxymethylfurfural + O2 | - |
Thermothelomyces thermophilus DSM 1799 | 2,5-diformylfuran + 5-formylfuran-2-carboxylic acid + H2O2 | - |
? | |
| anisyl alcohol + O2 | - |
Thermothelomyces thermophilus | anisyl aldehyde + H2O2 | - |
? | |
| anisyl alcohol + O2 | - |
Thermothelomyces thermophilus DSM 1799 | anisyl aldehyde + H2O2 | - |
? | |
| cinnamyl alcohol + O2 | - |
Thermothelomyces thermophilus | cinnamyl aldehyde + H2O2 | - |
? | |
| cinnamyl alcohol + O2 | - |
Thermothelomyces thermophilus DSM 1799 | cinnamyl aldehyde + H2O2 | - |
? | |
| additional information | no substrates: glucose, cellobiose, methanol | Thermothelomyces thermophilus | ? | - |
- |
|
| additional information | no substrates: glucose, cellobiose, methanol | Thermothelomyces thermophilus DSM 1799 | ? | - |
- |
|
| veratryl alcohol + O2 | - |
Thermothelomyces thermophilus | veratraldehyde + H2O2 | - |
? | |
| veratryl alcohol + O2 | - |
Thermothelomyces thermophilus DSM 1799 | veratraldehyde + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 68300, calculated from sequence | Thermothelomyces thermophilus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GMC oxidoreductase-like protein | - |
Thermothelomyces thermophilus |
| MYCTH_2299749 | - |
Thermothelomyces thermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Thermothelomyces thermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
half-life 10 min in absence of Ca2+, in presence of Ca2+ activity is maintained for 2 h | Thermothelomyces thermophilus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0008 | - |
5-hydroxymethylfurfural | pH 6, 30°C | Thermothelomyces thermophilus | |
| 0.0053 | - |
veratryl alcohol | pH 6, 30°C | Thermothelomyces thermophilus | |
| 0.0069 | - |
cinnamyl alcohol | pH 6, 30°C | Thermothelomyces thermophilus | |
| 0.0075 | - |
anisyl alcohol | pH 6, 30°C | Thermothelomyces thermophilus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Thermothelomyces thermophilus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | molar ration of FAD to purified enzyme is 0.69 | Thermothelomyces thermophilus | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.001 | - |
5-hydroxymethylfurfural | pH 6, 30°C | Thermothelomyces thermophilus | |
| 0.008 | - |
anisyl alcohol | pH 6, 30°C | Thermothelomyces thermophilus | |
| 0.011 | - |
veratryl alcohol | pH 6, 30°C | Thermothelomyces thermophilus | |
| 0.014 | - |
cinnamyl alcohol | pH 6, 30°C | Thermothelomyces thermophilus | |
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