Application | Comment | Organism |
---|---|---|
synthesis | expression and secretion of AAO with yield of 315 mg/l using Pichia pastoris | Pleurotus eryngii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
PAGE | Pleurotus eryngii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pleurotus eryngii | D3YBH4 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | about 60% glycosylation | Pleurotus eryngii |
Purification (Comment) | Organism |
---|---|
recombinant protein | Pleurotus eryngii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E)-hept-2-en-1-ol + O2 | 31.9% of the activity with benzyl alcohol | Pleurotus eryngii | (2E)-hept-2-enal + H2O2 | - |
? | |
(2E)-hex-2-en-1-ol + O2 | 63.9% of the activity with benzyl alcohol | Pleurotus eryngii | (2E)-hex-2-enal + H2O2 | - |
? | |
(2E,4E)-hepta-2,4-dien-1-ol + O2 | 737% of the activity with benzyl alcohol | Pleurotus eryngii | (2E,4E)-hepta-2,4-dienal + H2O2 | - |
? | |
(2E,4E)-hexa-2,4-dien-1-ol + O2 | 807% of the activity with benzyl alcohol | Pleurotus eryngii | (2E,4E)-hexa-2,4-dienal + H2O2 | - |
? | |
(2H-1,3-benzodioxol-5-yl)methanol + O2 | 301% of the activity with benzyl alcohol | Pleurotus eryngii | 2H-1,3-benzodioxole-5-carbaldehyde + H2O2 | - |
? | |
(naphthalen-2-yl)methanol + O2 | 874% of the activity with benzyl alcohol | Pleurotus eryngii | naphthalene-2-carbaldehyde + H2O2 | - |
? | |
(pyrene-1-yl)methanol + O2 | 35% of the activity with benzyl alcohol | Pleurotus eryngii | pyrene-1-carbaldehyde + H2O2 | - |
? | |
(thiophen-2-yl)methanol + O2 | 15.8% of the activity with benzyl alcohol | Pleurotus eryngii | thiophene-2-carbaldehyde + H2O2 | - |
? | |
4-aminobenzyl alcohol + O2 | 18.6% of the activity with benzyl alcohol | Pleurotus eryngii | 4-aminobenzaldehyde + H2O2 | - |
? | |
anisyl alcohol + O2 | 647% of the activity with benzyl alcohol | Pleurotus eryngii | anisyl aldehyde + H2O2 | - |
? | |
benzyl alcohol + O2 | - |
Pleurotus eryngii | benzaldehyde + H2O2 | - |
? | |
cinnamyl alcohol + O2 | 442% of the activity with benzyl alcohol | Pleurotus eryngii | cinnamyl aldehyde + H2O2 | - |
? | |
cumic alcohol + O2 | 149% of the activity with benzyl alcohol | Pleurotus eryngii | cumic aldehyde + H2O2 | - |
? | |
isovanillyl alcohol + O2 | 246% of the activity with benzyl alcohol | Pleurotus eryngii | isovanillyl aldehyde + H2O2 | - |
? | |
veratryl alcohol + O2 | 322% of the activity with benzyl alcohol | Pleurotus eryngii | veratryl aldehyde + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 100000, recombinant protein, 1 * 70000, PNGase F-treated protein, SDS-PAGE, 1 * 60800, calculated from sequence | Pleurotus eryngii |
Synonyms | Comment | Organism |
---|---|---|
AAO2 | - |
Pleurotus eryngii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 50 | 1 h, 90% residual activity | Pleurotus eryngii |
55 | - |
1 h, 70% residual activity | Pleurotus eryngii |
60 | - |
1 h, 10% residual activity | Pleurotus eryngii |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 9 | 1 h, 90% residual activity | Pleurotus eryngii |