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Literature summary for 1.1.3.4 extracted from
- Purification, properties, and molecular features of glucose oxidase from Aspergillus niger (1975), J. Biochem., 78, 835-843.
General Stability
| General Stability | Organism |
|---|---|
| SDS, 1.5%, 2-mercaptoethanol, 55°C, residual activity: 24% | Aspergillus niger |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | the enzyme contains 2 mol of iron per 160000 Da | Aspergillus niger |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 79000 | - |
2 * 79000, SDS-PAGE | Aspergillus niger |
| 158000 | 160000 | gel filtration | Aspergillus niger |
| 160000 | - |
gel filtration | Aspergillus niger |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-D-glucose + O2 + H2O | Aspergillus niger | - |
D-glucono-1,5-lactone + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | the enzyme contains 74% protein, 16.4% neutral sugar and 2.4% amino sugar, major component: mannose | Aspergillus niger |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from commercial preparation using DEAE-cellulose chromatography and Sephadex G-200 gel filtration | Aspergillus niger |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | - |
Aspergillus niger | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 172 | - |
purified enzyme | Aspergillus niger |
Storage Stability
| Storage Stability | Organism |
|---|---|
| lyophilized, over P2O5 | Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-D-glucose + O2 + H2O | - |
Aspergillus niger | D-glucono-1,5-lactone + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 79000, SDS-PAGE | Aspergillus niger |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Aspergillus niger | |
| Flavin-hypoxanthine dinucleotide | FHD, can substitute FAD | Aspergillus niger | |
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