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Literature summary for 1.1.3.4 extracted from

  • Szajani, B.; Molnar, A.; Klamar, G.; Kalman, M.
    Preparation, characterization, and potential application of an immobilized glucose oxidase (1987), Appl. Biochem. Biotechnol., 14, 37-47.
    View publication on PubMed

Application

Application Comment Organism
analysis immobilized enzyme on polyacrylamide employed for the determination of glucose concentration in blood sera Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
33
-
beta-D-glucose soluble enzyme Aspergillus niger
44
-
beta-D-glucose immobilized enzyme Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-D-glucose + O2 + H2O Aspergillus niger
-
D-glucono-1,5-lactone + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-

Storage Stability

Storage Stability Organism
4°C, immobilized, 11% loss of activity, 1 year Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-glucose + O2 + H2O
-
Aspergillus niger D-glucono-1,5-lactone + H2O2
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
immobilized on polyacrylamide Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
up to, soluble enzyme Aspergillus niger
50
-
up to, immobilized enzyme Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
immobilized on polyacrylamide Aspergillus niger

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5
-
50°C, most stable, immobilized enzyme Aspergillus niger
6
-
50°C, most stable at, soluble enzyme Aspergillus niger