Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.3.4 extracted from

  • Takegawa, K.; Fujiwara, K.; Iwahara, S.; Yamamoto, K.; Tochikura, T.
    Effect of deglycosylation of N-linked sugar chains on glucose oxidase from Aspergillus niger (1989), Biochem. Cell Biol., 67, 460-464.
    View publication on PubMed
Search for more literature for 1.1.3.4

General Stability

General Stability Organism
freezing/thawing, stable Aspergillus niger
SDS, 5%, stable to Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4 5.4 beta-D-glucose immobilized enzyme Aspergillus niger
37
-
 beta-D-glucose native enzyme Aspergillus niger
37 38 beta-D-glucose
-
 Aspergillus niger
38
-
 beta-D-glucose carbohydrate-depleted enzyme Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
87100
-
 x * 87100, SDS-PAGE Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-D-glucose + O2 + H2O Aspergillus niger
-
 D-glucono-1,5-lactone + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein N- and O-linked sugar chains, the enzyme contains 15.2% carbohydrates Aspergillus niger
additional information endo-beta-N-acetylglucosaminidase from Flavobacterium sp. releases about 30% of the N-linked sugar chains from the enzyme Aspergillus niger

Purification (Commentary)

Purification (Comment) Organism
purchased and further purified by DEAE-Toyopearl 650M column chromatography Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-glucose + O2 + H2O
-
 Aspergillus niger D-glucono-1,5-lactone + H2O2
-
 ?

Subunits

Subunits Comment Organism
? x * 87100, SDS-PAGE Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 native and carbohydrate-depleted enzyme, no decrease of activity after 100 freeze-thaw cycles Aspergillus niger
50
-
 both native and carbonhydrate-depleted enzyme retain full activity below Aspergillus niger
55
-
 native and carbohydrate-depleted enzyme, inactivation above Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 6 native and deglycosylated enzyme Aspergillus niger

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 8 native and deglycosylated form Aspergillus niger