Protein Variants | Comment | Organism |
---|---|---|
S101A | stopped-flow traces for the reductive half-reaction are monophasic like wild-type choline oxidase | Arthrobacter globiformis |
S101C | contrary to wild-type, stopped-flow traces for the reductive half-reaction are biphasic, corresponding to the reactions of proton abstraction and hydride transfer. The rate constants for proton transfer in the S101T/C/V variants decrease logarithmically with increasing hydrophobicity of residue 101 | Arthrobacter globiformis |
S101T | contrary to wild-type, stopped-flow traces for the reductive half-reaction are biphasic, corresponding to the reactions of proton abstraction and hydride transfer. The rate constants for proton transfer in the S101T/C/V variants decrease logarithmically with increasing hydrophobicity of residue 101 | Arthrobacter globiformis |
S101V | contrary to wild-type, stopped-flow traces for the reductive half-reaction are biphasic, corresponding to the reactions of proton abstraction and hydride transfer. The rate constants for proton transfer in the S101T/C/V variants decrease logarithmically with increasing hydrophobicity of residue 101 | Arthrobacter globiformis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
O2 | mutant S101V, pH 10, 25°C | Arthrobacter globiformis | |
0.014 | - |
O2 | mutant S101C, pH 10, 25°C | Arthrobacter globiformis | |
0.11 | - |
Betaine aldehyde | mutant S101C, pH 10, 25°C | Arthrobacter globiformis | |
0.21 | - |
O2 | mutant S101A, pH 10, 25°C | Arthrobacter globiformis | |
0.21 | - |
Betaine aldehyde | mutant S101T, pH 10, 25°C | Arthrobacter globiformis | |
0.48 | - |
O2 | mutant S101T, pH 10, 25°C | Arthrobacter globiformis | |
0.54 | - |
Betaine aldehyde | mutant S101V, pH 10, 25°C | Arthrobacter globiformis | |
2 | - |
O2 | wild-type, pH 10, 25°C | Arthrobacter globiformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter globiformis | Q7X2H8 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
betaine aldehyde + O2 + H2O | - |
Arthrobacter globiformis | betaine + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
codA | - |
Arthrobacter globiformis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
Betaine aldehyde | mutant S101V, pH 10, 25°C | Arthrobacter globiformis | |
0.7 | - |
Betaine aldehyde | mutant S101C, pH 10, 25°C | Arthrobacter globiformis | |
21 | - |
Betaine aldehyde | mutant S101T, pH 10, 25°C | Arthrobacter globiformis | |
42 | - |
Betaine aldehyde | mutant S101A, pH 10, 25°C | Arthrobacter globiformis | |
133 | - |
Betaine aldehyde | wild-type, pH 10, 25°C | Arthrobacter globiformis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.38 | - |
Betaine aldehyde | mutant S101V, pH 10, 25°C | Arthrobacter globiformis | |
6.1 | - |
Betaine aldehyde | mutant S101C, pH 10, 25°C | Arthrobacter globiformis | |
32 | - |
O2 | mutant S101V, pH 10, 25°C | Arthrobacter globiformis | |
43 | - |
O2 | mutant S101T, pH 10, 25°C | Arthrobacter globiformis | |
48 | - |
O2 | mutant S101C, pH 10, 25°C | Arthrobacter globiformis | |
53 | - |
O2 | wild-type, pH 10, 25°C | Arthrobacter globiformis | |
99 | - |
Betaine aldehyde | mutant S101T, pH 10, 25°C | Arthrobacter globiformis | |
203 | - |
O2 | mutant S101A, pH 10, 25°C | Arthrobacter globiformis |