Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta(DE3)pLysS cells | Arthrobacter globiformis |
Protein Variants | Comment | Organism |
---|---|---|
F357A | the mutant shows about 240fold reduced catalytic efficiency compared to the wild type enzyme | Arthrobacter globiformis |
M62A | the mutant shows about 3fold reduced catalytic efficiency compared to the wild type enzyme | Arthrobacter globiformis |
M62A/F357A | the enzyme shows a lack of the isomerization detected in wild type choline oxidase, and a lack of saturation with an oxygen concentration as high as 1 mM, while most other kinetic parameters are similar to those of wild type choline oxidase | Arthrobacter globiformis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
choline | wild type enzyme, at pH 7.0 and 25°C | Arthrobacter globiformis | |
0.8 | - |
choline | mutant enzyme M62A, at pH 7.0 and 25°C | Arthrobacter globiformis | |
9 | - |
choline | mutant enzyme M62A/F357A, at pH 7.0 and 25°C | Arthrobacter globiformis | |
12 | - |
choline | mutant enzyme F357A, at pH 7.0 and 25°C | Arthrobacter globiformis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
betaine aldehyde + O2 + H2O | Arthrobacter globiformis | - |
betaine + H2O2 | - |
? | |
choline + O2 | Arthrobacter globiformis | - |
betaine aldehyde + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter globiformis | Q7X2H8 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
betaine aldehyde + O2 + H2O | - |
Arthrobacter globiformis | betaine + H2O2 | - |
? | |
choline + O2 | - |
Arthrobacter globiformis | betaine aldehyde + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
choline:oxygen 1-reductase | - |
Arthrobacter globiformis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.5 | - |
choline | mutant enzyme F357A, at pH 7.0 and 25°C | Arthrobacter globiformis | |
6.8 | - |
choline | mutant enzyme M62A/F357A, at pH 7.0 and 25°C | Arthrobacter globiformis | |
12.5 | - |
choline | mutant enzyme M62A, at pH 7.0 and 25°C | Arthrobacter globiformis | |
20 | - |
choline | wild type enzyme, at pH 7.0 and 25°C | Arthrobacter globiformis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Arthrobacter globiformis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.21 | - |
choline | mutant enzyme F357A, at pH 7.0 and 25°C | Arthrobacter globiformis | |
0.8 | - |
choline | mutant enzyme M62A/F357A, at pH 7.0 and 25°C | Arthrobacter globiformis | |
16 | - |
choline | mutant enzyme M62A, at pH 7.0 and 25°C | Arthrobacter globiformis | |
50 | - |
choline | wild type enzyme, at pH 7.0 and 25°C | Arthrobacter globiformis |