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Literature summary for 1.1.3.17 extracted from

  • Smitherman, C.; Rungsrisuriyachai, K.; Germann, M.W.; Gadda, G.
    Identification of the catalytic base for alcohol activation in choline oxidase (2015), Biochemistry, 54, 413-421.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta(DE3)pLysS cells Arthrobacter globiformis

Protein Variants

Protein Variants Comment Organism
H351Q the kcat and kcat/Km values of the H351Q emutant in atmospheric oxygen are 45 and 5000fold lower than those of the wild type enzyme, respectively Arthrobacter globiformis
H466Q inactive Arthrobacter globiformis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.6
-
O2 wild type enzyme, in 50 mM potassium phosphate (pH 7.0), at 25°C Arthrobacter globiformis
67
-
O2 mutant enzyme H351Q, in 50 mM potassium phosphate (pH 7.0), at 25°C Arthrobacter globiformis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
betaine aldehyde + O2 + H2O Arthrobacter globiformis
-
glycine betaine + H2O2
-
?
choline + O2 Arthrobacter globiformis
-
betaine aldehyde + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Arthrobacter globiformis Q7X2H8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
betaine aldehyde + O2 + H2O
-
Arthrobacter globiformis glycine betaine + H2O2
-
?
choline + O2
-
Arthrobacter globiformis betaine aldehyde + H2O2
-
?

Synonyms

Synonyms Comment Organism
CHO
-
Arthrobacter globiformis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.33
-
O2 mutant enzyme H351Q, in 50 mM potassium phosphate (pH 7.0), at 25°C Arthrobacter globiformis
15
-
O2 wild type enzyme, in 50 mM potassium phosphate (pH 7.0), at 25°C Arthrobacter globiformis

Cofactor

Cofactor Comment Organism Structure
FAD
-
Arthrobacter globiformis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0049
-
O2 mutant enzyme H351Q, in 50 mM potassium phosphate (pH 7.0), at 25°C Arthrobacter globiformis
25
-
O2 wild type enzyme, in 50 mM potassium phosphate (pH 7.0), at 25°C Arthrobacter globiformis