Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta(DE3)pLysS cells | Arthrobacter globiformis |
Protein Variants | Comment | Organism |
---|---|---|
H351Q | the kcat and kcat/Km values of the H351Q emutant in atmospheric oxygen are 45 and 5000fold lower than those of the wild type enzyme, respectively | Arthrobacter globiformis |
H466Q | inactive | Arthrobacter globiformis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
O2 | wild type enzyme, in 50 mM potassium phosphate (pH 7.0), at 25°C | Arthrobacter globiformis | |
67 | - |
O2 | mutant enzyme H351Q, in 50 mM potassium phosphate (pH 7.0), at 25°C | Arthrobacter globiformis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
betaine aldehyde + O2 + H2O | Arthrobacter globiformis | - |
glycine betaine + H2O2 | - |
? | |
choline + O2 | Arthrobacter globiformis | - |
betaine aldehyde + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter globiformis | Q7X2H8 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
betaine aldehyde + O2 + H2O | - |
Arthrobacter globiformis | glycine betaine + H2O2 | - |
? | |
choline + O2 | - |
Arthrobacter globiformis | betaine aldehyde + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CHO | - |
Arthrobacter globiformis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.33 | - |
O2 | mutant enzyme H351Q, in 50 mM potassium phosphate (pH 7.0), at 25°C | Arthrobacter globiformis | |
15 | - |
O2 | wild type enzyme, in 50 mM potassium phosphate (pH 7.0), at 25°C | Arthrobacter globiformis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Arthrobacter globiformis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0049 | - |
O2 | mutant enzyme H351Q, in 50 mM potassium phosphate (pH 7.0), at 25°C | Arthrobacter globiformis | |
25 | - |
O2 | wild type enzyme, in 50 mM potassium phosphate (pH 7.0), at 25°C | Arthrobacter globiformis |