Crystallization (Comment) | Organism |
---|---|
- |
Gluconobacter oxydans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | - |
Gluconobacter oxydans | |
PCMB | - |
Gluconobacter oxydans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
NADPH | - |
Gluconobacter oxydans | |
0.02 | - |
NADP+ | - |
Gluconobacter oxydans | |
0.9 | - |
5-Dehydro-D-gluconate | - |
Gluconobacter oxydans | |
20 | - |
D-gluconate | - |
Gluconobacter oxydans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
4 * 25000, SDS-PAGE | Gluconobacter oxydans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-dehydro-D-gluconate + NADPH | Gluconobacter oxydans | the suggested physiological role of the enzyme is to reduce 5-dehydro-D-gluconate to D-gluconate to supply a carbon source and regenerate NADP+ | D-gluconate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconobacter oxydans | - |
IFO 12528 | - |
Purification (Comment) | Organism |
---|---|
- |
Gluconobacter oxydans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
69.69 | - |
- |
Gluconobacter oxydans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-dehydro-D-gluconate + NADPH | - |
Gluconobacter oxydans | D-gluconate + NADP+ | - |
? | |
5-dehydro-D-gluconate + NADPH | the suggested physiological role of the enzyme is to reduce 5-dehydro-D-gluconate to D-gluconate to supply a carbon source and regenerate NADP+ | Gluconobacter oxydans | D-gluconate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 25000, SDS-PAGE | Gluconobacter oxydans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
reduction of 5-dehydro-D-gluconate and oxidation of D-gluconate | Gluconobacter oxydans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
thermal stability is enhanced by addition of either D-gluconate or 5-keto-D-gluconate, or both | Gluconobacter oxydans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
reduction of 5-dehydro-D-gluconate | Gluconobacter oxydans |
10 | - |
oxidation of D-gluconate in presence of NADP+ | Gluconobacter oxydans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | cofactor | Gluconobacter oxydans |