Cloned (Comment) | Organism |
---|---|
sequence homology with other NADPH-dependent oxidoreductases from plants | Nicotiana tabacum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
2 * 37000, SDS-PAGE and amino acid sequence analysis | Nicotiana tabacum |
74000 | - |
gel filtration | Nicotiana tabacum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
allyl alcohol + NADP+ | Nicotiana tabacum | - |
acrolein + NADPH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Nicotiana tabacum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.11 | - |
- |
Nicotiana tabacum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S,4S)-carveol + NADP+ | - |
Nicotiana tabacum | (S)-carvone + NADPH | - |
r | |
(S)-carvone + NADPH | - |
Nicotiana tabacum | (2S,4S)-carveol + NADP+ | - |
r | |
allyl alcohol + NADP+ | - |
Nicotiana tabacum | acrolein + NADPH | - |
r | |
allyl alcohol + NADP+ | specific for the (S)-isomer of secondary allylic alcohols, several stereomers tested for activity in both reaction directions | Nicotiana tabacum | acrolein + NADPH | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 37000, SDS-PAGE and amino acid sequence analysis | Nicotiana tabacum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
reduction of carbonyl group of (S)-carvone | Nicotiana tabacum |
8 | - |
dehydrogenation of (S)-carveol | Nicotiana tabacum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD(P)H | - |
Nicotiana tabacum | |
NADP+ | activity 4times greater than with NAD+ | Nicotiana tabacum |