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Literature summary for 1.1.1.49 extracted from
- Purification and kinetics of sheep kidney cortex glucose-6-phosphate dehydrogenase (2006), Comp. Biochem. Physiol. B, 143B, 249-255.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | ping pong bi bi kinetic mechanism | Ovis aries | |
| 0.0147 | - |
NADP+ | pH 7.4, 37°C | Ovis aries |  |
| 0.041 | - |
D-glucose 6-phosphate | pH 7.4, 37°C | Ovis aries | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Ovis aries | 5829 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose 6-phosphate + NADP+ + H2O | Ovis aries | the enzyme is responsible for the production of NADPH, which is required for biosynthetic reactions and protection of cells from free radicals | D-gluconate 6-phosphate + NADPH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ovis aries | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 1384fold from kidney cortex by ultracentrifugation, 2',5'-ADP affinity and anion exchange chromatography | Ovis aries |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH + H+ | the adult kidney cortex enzyme shows a ping pong bi bi kinetic mechanism, while the lamb kidney enzyme obeys an ordered bi bi mechanism, thus the kinetic mechanism alters due to aging | Ovis aries |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney cortex | - |
Ovis aries | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 27.69 | - |
purified kidney cortex enzyme | Ovis aries |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose 6-phosphate + NADP+ + H2O | - |
Ovis aries | D-gluconate 6-phosphate + NADPH + H+ | - |
? | |
| D-glucose 6-phosphate + NADP+ + H2O | the enzyme is responsible for the production of NADPH, which is required for biosynthetic reactions and protection of cells from free radicals | Ovis aries | D-gluconate 6-phosphate + NADPH + H+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| G-6-PD | - |
Ovis aries |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Ovis aries |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 60 | temperature profile | Ovis aries |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
- |
Ovis aries |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | pH profile | Ovis aries |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 10 | stable, purified enzyme | Ovis aries |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Ovis aries | |
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Release 2023.1 (January 2023)
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