Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli. The expressed enzyme contains a 6-histidine tag at the N-terminus | Lysinibacillus xylanilyticus |
Protein Variants | Comment | Organism |
---|---|---|
A164F | mutation improves the specific activity of the enzyme toward methanol compared to that of the wild-type enzyme. Mutant shows a slightly higher turnover rate than that of wild-type, although the KM value is increased compared to that of wild-type | Lysinibacillus xylanilyticus |
S101V | mutation improves the specific activity of the enzyme toward methanol compared to that of the wild-type enzyme. Mutant shows a slightly higher turnover rate than that of wild-type, although the KM value is increased compared to that of wild-type | Lysinibacillus xylanilyticus |
T141S | mutation improves the specific activity of the enzyme toward methanol compared to that of the wild-type enzyme. Mutant shows a slightly higher turnover rate than that of wild-type, although the KM value is increased compared to that of wild-type | Lysinibacillus xylanilyticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | - |
Lysinibacillus xylanilyticus | |
Cu2+ | - |
Lysinibacillus xylanilyticus | |
Zn2+ | - |
Lysinibacillus xylanilyticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
NAD+ | pH 9.5, 55°C, wild-type enzyme | Lysinibacillus xylanilyticus | |
0.25 | - |
ethanol | pH 9.5, 55°C, wild-type enzyme | Lysinibacillus xylanilyticus | |
0.5 | - |
butanol | pH 9.5, 55°C, wild-type enzyme | Lysinibacillus xylanilyticus | |
1.09 | - |
n-Propanol | pH 9.5, 55°C, wild-type enzyme | Lysinibacillus xylanilyticus | |
1.19 | - |
ethanol | pH 9.5, 55°C, mutant enzyme A164F | Lysinibacillus xylanilyticus | |
1.38 | - |
ethanol | pH 9.5, 55°C, mutant enzyme T141S | Lysinibacillus xylanilyticus | |
1.82 | - |
n-Propanol | pH 9.5, 55°C, mutant enzyme T141S | Lysinibacillus xylanilyticus | |
2.33 | - |
n-Propanol | pH 9.5, 55°C, mutant enzyme A164F | Lysinibacillus xylanilyticus | |
3.23 | - |
methanol | pH 9.5, 55°C, wild-type enzyme | Lysinibacillus xylanilyticus | |
9.45 | - |
n-Propanol | pH 9.5, 55°C, mutant enzyme S101V | Lysinibacillus xylanilyticus | |
10.35 | - |
methanol | pH 9.5, 55°C, mutant enzyme S101V | Lysinibacillus xylanilyticus | |
17.38 | - |
ethanol | pH 9.5, 55°C, mutant enzyme S101V | Lysinibacillus xylanilyticus | |
36.83 | - |
methanol | pH 9.5, 55°C, mutant enzyme A164F | Lysinibacillus xylanilyticus | |
51.24 | - |
methanol | pH 9.5, 55°C, mutant enzyme T141S | Lysinibacillus xylanilyticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 10 mM, strong activation | Lysinibacillus xylanilyticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
420000 | - |
gel filtration | Lysinibacillus xylanilyticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
methanol + NAD+ | Lysinibacillus xylanilyticus | - |
formaldehyde + NADH + H+ | - |
? | |
methanol + NAD+ | Lysinibacillus xylanilyticus KCTC 13423 | - |
formaldehyde + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lysinibacillus xylanilyticus | - |
- |
- |
Lysinibacillus xylanilyticus KCTC 13423 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Lysinibacillus xylanilyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
butanol + NAD+ | - |
Lysinibacillus xylanilyticus | butyraldehyde + NADH + H+ | - |
? | |
butanol + NAD+ | - |
Lysinibacillus xylanilyticus KCTC 13423 | butyraldehyde + NADH + H+ | - |
? | |
ethanol + NAD+ | - |
Lysinibacillus xylanilyticus | acetaldehyde + NADH + H+ | - |
? | |
ethanol + NAD+ | - |
Lysinibacillus xylanilyticus KCTC 13423 | acetaldehyde + NADH + H+ | - |
? | |
methanol + NAD+ | - |
Lysinibacillus xylanilyticus | formaldehyde + NADH + H+ | - |
? | |
methanol + NAD+ | - |
Lysinibacillus xylanilyticus KCTC 13423 | formaldehyde + NADH + H+ | - |
? | |
n-propanol + NAD+ | - |
Lysinibacillus xylanilyticus | propionaldehyde + NADH + H+ | - |
? | |
n-propanol + NAD+ | - |
Lysinibacillus xylanilyticus KCTC 13423 | propionaldehyde + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
decamer | 10 * 42800, SDS-PAGE | Lysinibacillus xylanilyticus |
Synonyms | Comment | Organism |
---|---|---|
lxmdh | - |
Lysinibacillus xylanilyticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
in the presence of 10 mM Mg2+ | Lysinibacillus xylanilyticus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 70 | 35°C: about 40% of maximal activity, 70°C: about 50% of maximal activity | Lysinibacillus xylanilyticus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.21 | - |
methanol | pH 9.5, 55°C, wild-type enzyme | Lysinibacillus xylanilyticus | |
0.24 | - |
methanol | pH 9.5, 55°C, mutant enzyme S101V | Lysinibacillus xylanilyticus | |
0.31 | - |
n-Propanol | pH 9.5, 55°C, mutant enzyme S101V | Lysinibacillus xylanilyticus | |
0.33 | - |
methanol | pH 9.5, 55°C, mutant enzyme A164F | Lysinibacillus xylanilyticus | |
0.33 | - |
methanol | pH 9.5, 55°C, mutant enzyme T141S | Lysinibacillus xylanilyticus | |
0.37 | - |
NAD+ | pH 9.5, 55°C, wild-type enzyme | Lysinibacillus xylanilyticus | |
0.46 | - |
ethanol | pH 9.5, 55°C, wild-type enzyme | Lysinibacillus xylanilyticus | |
0.52 | - |
butanol | pH 9.5, 55°C, wild-type enzyme | Lysinibacillus xylanilyticus | |
0.55 | - |
n-Propanol | pH 9.5, 55°C, wild-type enzyme | Lysinibacillus xylanilyticus | |
0.59 | - |
ethanol | pH 9.5, 55°C, mutant enzyme S101V | Lysinibacillus xylanilyticus | |
0.62 | - |
ethanol | pH 9.5, 55°C, mutant enzyme A164F | Lysinibacillus xylanilyticus | |
0.65 | - |
ethanol | pH 9.5, 55°C, mutant enzyme T141S | Lysinibacillus xylanilyticus | |
0.69 | - |
n-Propanol | pH 9.5, 55°C, mutant enzyme T141S | Lysinibacillus xylanilyticus | |
0.79 | - |
n-Propanol | pH 9.5, 55°C, mutant enzyme A164F | Lysinibacillus xylanilyticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
in the presence of 10 mM Mg2+ | Lysinibacillus xylanilyticus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8.5 | 10 | pH 8.5: about 50% of maximal activity, pH 10.0: about 90% of maximal activity | Lysinibacillus xylanilyticus |
General Information | Comment | Organism |
---|---|---|
metabolism | methanol oxidation catalyzed by methanol dehydrogenase is one of the key steps in methanol utilization in bacterial methylotrophy | Lysinibacillus xylanilyticus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0064 | - |
methanol | pH 9.5, 55°C, mutant enzyme T141S | Lysinibacillus xylanilyticus | |
0.0092 | - |
methanol | pH 9.5, 55°C, mutant enzyme A164F | Lysinibacillus xylanilyticus | |
0.0236 | - |
methanol | pH 9.5, 55°C, mutant enzyme S101V | Lysinibacillus xylanilyticus | |
0.0335 | - |
n-Propanol | pH 9.5, 55°C, mutant enzyme S101V | Lysinibacillus xylanilyticus | |
0.0339 | - |
ethanol | pH 9.5, 55°C, mutant enzyme S101V | Lysinibacillus xylanilyticus | |
0.0668 | - |
methanol | pH 9.5, 55°C, wild-type enzyme | Lysinibacillus xylanilyticus | |
0.34 | - |
n-Propanol | pH 9.5, 55°C, mutant enzyme A164F | Lysinibacillus xylanilyticus | |
0.381 | - |
n-Propanol | pH 9.5, 55°C, mutant enzyme T141S | Lysinibacillus xylanilyticus | |
0.474 | - |
ethanol | pH 9.5, 55°C, mutant enzyme T141S | Lysinibacillus xylanilyticus | |
0.509 | - |
n-Propanol | pH 9.5, 55°C, wild-type enzyme | Lysinibacillus xylanilyticus | |
0.523 | - |
ethanol | pH 9.5, 55°C, mutant enzyme A164F | Lysinibacillus xylanilyticus | |
1.048 | - |
butanol | pH 9.5, 55°C, wild-type enzyme | Lysinibacillus xylanilyticus | |
1.648 | - |
NAD+ | pH 9.5, 55°C, wild-type enzyme | Lysinibacillus xylanilyticus | |
1.861 | - |
ethanol | pH 9.5, 55°C, wild-type enzyme | Lysinibacillus xylanilyticus |