Application | Comment | Organism |
---|---|---|
analysis | quantitative determination of N-acetylneuraminic acid | Flavobacterium sp. |
Cloned (Comment) | Organism |
---|---|
cloned and expressed under control of a lac-promoter in Escherichia coli JM109, the Escherichia coli transformants JM109(pNAM307) and JM109(pNAM308) show up to 200-fold higher activity than Flavobacterium sp. | Flavobacterium sp. |
General Stability | Organism |
---|---|
inactivated by frequent freezing and thawing | Flavobacterium sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | slight inhibition | Flavobacterium sp. | |
Cd2+ | slight inhibition | Flavobacterium sp. | |
Cu2+ | slight inhibition | Flavobacterium sp. | |
Hg2+ | strong inhibitor | Flavobacterium sp. | |
Mn2+ | slight inhibition | Flavobacterium sp. | |
additional information | no inhibition with metal-chelating or SH-group-blocking reagents, e.g. EDTA, 2,2'-bipyridyl, 8-hydroxyquinoline, PCMB | Flavobacterium sp. | |
Ni2+ | slight inhibition | Flavobacterium sp. | |
SDS | strong inhibitor | Flavobacterium sp. | |
Zn2+ | slight inhibition | Flavobacterium sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.41 | - |
NAD+ | - |
Flavobacterium sp. | |
1 | - |
N-acetyl-D-mannosamine | - |
Flavobacterium sp. | |
13.3 | - |
N-glycolyl-D-mannosamine | - |
Flavobacterium sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27470 | - |
calculated from amino acid sequence | Flavobacterium sp. |
29000 | - |
SDS-PAGE | Flavobacterium sp. |
120000 | - |
gel filtration | Flavobacterium sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-D-mannosamine + NAD+ | Flavobacterium sp. | reaction in N-acetyl-D-mannosamine catabolism | N-acetyl-D-mannosaminolactone + NADH + H+ | - |
? | |
N-acetyl-D-mannosamine + NAD+ | Flavobacterium sp. 141-8 | reaction in N-acetyl-D-mannosamine catabolism | N-acetyl-D-mannosaminolactone + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Flavobacterium sp. | - |
141-8 | - |
Flavobacterium sp. 141-8 | - |
141-8 | - |
Purification (Comment) | Organism |
---|---|
- |
Flavobacterium sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.13 | - |
crude extract | Flavobacterium sp. |
340 | - |
purified enzyme | Flavobacterium sp. |
Storage Stability | Organism |
---|---|
-20°C, stable for at least several months | Flavobacterium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-D-mannosamine + NAD+ | - |
Flavobacterium sp. | N-acetyl-D-mannosaminolactone + NADH + H+ | - |
ir | |
N-acetyl-D-mannosamine + NAD+ | reaction in N-acetyl-D-mannosamine catabolism | Flavobacterium sp. | N-acetyl-D-mannosaminolactone + NADH + H+ | - |
? | |
N-acetyl-D-mannosamine + NAD+ | - |
Flavobacterium sp. 141-8 | N-acetyl-D-mannosaminolactone + NADH + H+ | - |
ir | |
N-acetyl-D-mannosamine + NAD+ | reaction in N-acetyl-D-mannosamine catabolism | Flavobacterium sp. 141-8 | N-acetyl-D-mannosaminolactone + NADH + H+ | - |
? | |
N-glycolyl-D-mannosamine + NAD+ | oxidation at 62% the rate of N-acetyl-D-mannosamine oxidation | Flavobacterium sp. | N-glycolyl-D-mannosaminolactone + NADH | - |
? | |
N-glycolyl-D-mannosamine + NAD+ | oxidation at 62% the rate of N-acetyl-D-mannosamine oxidation | Flavobacterium sp. 141-8 | N-glycolyl-D-mannosaminolactone + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 29000 | Flavobacterium sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
inactivation at pH 5.0 after 16 h, gradually reversed to about 80% of original activity by shifting the pH back to 8.2 and keeping it at room temperature for 36 h | Flavobacterium sp. |
42 | - |
and below, stable for at least 10 min | Flavobacterium sp. |
50 | - |
t1/2: 10 min | Flavobacterium sp. |
60 | - |
complete inactivation after 10 min | Flavobacterium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9.5 | - |
Flavobacterium sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 11.5 | - |
Flavobacterium sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
inactivation at 25°C after 16 h, gradually reversed to about 80% of original activity by shifting the pH back to 8.2 and keeping it at room temperature for 36 h | Flavobacterium sp. |
7 | - |
and below, more than 70% loss of activity | Flavobacterium sp. |
8.5 | 9.5 | more stable than at pH 8.2 at 45°C, 10 min | Flavobacterium sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no cofactor: NADP+, ferricyanide, 2,6-dichlorophenolindophenol, phenazine methosulfate | Flavobacterium sp. | |
NAD+ | specific for | Flavobacterium sp. |