Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NADH | 0.05 mM, partial inhibition | Capra hircus | |
UDP | 0.5 mM, partial inhibition | Capra hircus | |
UDP-alpha-D-glucuronate | 0.05 mM, partial inhibition | Capra hircus | |
UDP-galactose | 0.5 mM, partial inhibition | Capra hircus | |
UDP-xylose | 0.05 mM, partial inhibition | Capra hircus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
UDP-alpha-D-glucose | pH 8.8, 25°C | Capra hircus | |
0.132 | - |
NAD+ | pH 8.8, 25°C | Capra hircus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
6 * 50000, SDS-PAGE | Capra hircus |
300000 | - |
gel filtration | Capra hircus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Capra hircus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Capra hircus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Capra hircus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
342.9 | - |
pH 8.8, 25°C | Capra hircus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no substrates: UDP-D-galactose, UTP, 5'-UMP and D-galactose | Capra hircus | ? | - |
? | |
UDP-alpha-D-glucose + 2 NAD+ + H2O | - |
Capra hircus | UDP-alpha-D-glucuronate + 2 NADH + 2 H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 50000, SDS-PAGE | Capra hircus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 45 | - |
Capra hircus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | - |
- |
Capra hircus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8.8 | - |
80-83% of maximum activity | Capra hircus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.4 | 6.5 | enzyme retains 93-97% of initial activity | Capra hircus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Capra hircus | |
NADP+ | about 5-12% of the activity with NAD+ | Capra hircus |