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Literature summary for 1.1.1.22 extracted from

  • Wei, Q.; Galbenus, R.; Raza, A.; Cerny, R.L.; Simpson, M.A.
    Androgen-stimulated UDP-glucose dehydrogenase expression limits prostate androgen availability without impacting hyaluronan levels (2009), Cancer Res., 69, 2332-2339.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
hUGDH gene is cloned from a LNCaP cDNA library, from LNCaP C33 and C81 cells, expression of His6-tagged UGDH in Escherichia coli, expression of UGDH mutant D280N in HEK.293 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
D280N an inactive UGDH mutant Homo sapiens
additional information UGDH overexpression stimulates hyaluronan production in HEK293 cells Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-glucose + 2 NAD+ + H2O Homo sapiens
-
UDP-glucuronic acid + 2 NADH + 2 H+
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged UGDH from Escherichia coli by nickel affinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver high expression level Homo sapiens
-
LNCaP cell
-
Homo sapiens
-
PC-3M-LN4 cell prostate tumor cell line derived from PC-3 cells Homo sapiens
-
prostate high expression level Homo sapiens
-
prostate cancer cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-glucose + 2 NAD+ + H2O
-
Homo sapiens UDP-glucuronic acid + 2 NADH + 2 H+
-
?

Synonyms

Synonyms Comment Organism
UDP-glucose dehydrogenase
-
Homo sapiens
UGDH
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Homo sapiens

Expression

Organism Comment Expression
Homo sapiens dihydrotestosterone increases UGDH expression 2.5fold in androgen-dependent cells. However, upregulation of UGDH does not affect hyaluronan synthase expression or enhance hyaluronan production up

General Information

General Information Comment Organism
additional information dysregulated expression of UGDH can promote the development of androgen independent tumor cell growth by increasing available levels of intracellular androgen. UGDH activity is the rate limiting factor in solubilization of excess androgen from prostate tumor cells, overview Homo sapiens
physiological function UGDH oxidizes UDP-glucose to UDP-glucuronate, an essential precursor for production of hyaluronan, proteoglycans, and xenobiotic glucuronides. High levels of hyaluronan turnover in prostate cancer are correlated with aggressive progression. UGDH expression is high in the normal prostate even though hyaluronan accumulation is virtually undetectable. The enzyme's common role in the prostate may be to provide precursors for glucuronosyltransferase enzymes, which inactivate and solubilize androgens by glucuronidation. Androgen dependence of UGDH, glucuronosyltransferase, and hyaluronan synthase expression, overview Homo sapiens