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Literature summary for 1.1.1.22 extracted from

  • Rocha, J.; Granja, A.T.; Sa-Correia, I.; Fialho, A.; Frazao, C.
    Cloning, expression, purification, crystallization and preliminary crystallographic studies of UgdG, an UDP-glucose dehydrogenase from Sphingomonas elodea ATCC 31461 (2010), Acta Crystallogr. Sect. F, 66, 69-72.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene ugdG, expression of His6-tagged wild-type and selenomethionine-labeled UgdG in Escherichia coli BL21 and B843, respectively Sphingomonas elodea

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant wild-type and selenomethionine-labeled UgdG at 4.5 and 5.5 mg/ml, respectively, in 25 mM Tris-HCl, pH 8.3, 50 mM NaCl, 2.5 mM DTT and 1 mM NAD+, or 0.5 mM UDP-GlcA and 1 mM NAD+, 0.0005 ml of each protein and precipitant solution are mixed at 20°C, 24 h, the precipitant solution contains 200 mM Li2SO4, 100 mM Tris-HCl, pH 8.5, and 30% v/v PEG 4000, or 100 mM sodium citrate, pH 5.6, 20% 2-propanol, and 20% v/v PEG 4000, vapour diffusion method, method optimization, X-ray diffraction structure determination and analysis at 2.4 A resolution for the wild-type enzyme, and at 3.4 A resolution for the SeMet-UDG Sphingomonas elodea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-glucose + 2 NAD+ + H2O Sphingomonas elodea
-
UDP-glucuronic acid + 2 NADH + 2 H+
-
?
UDP-glucose + 2 NAD+ + H2O Sphingomonas elodea ATCC 31461
-
UDP-glucuronic acid + 2 NADH + 2 H+
-
?

Organism

Organism UniProt Comment Textmining
Sphingomonas elodea A4UTT2 gene ugdG
-
Sphingomonas elodea ATCC 31461 A4UTT2 gene ugdG
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged wild-type and selenomethionine-labeled UgdG from Escherichia coli BL21 and B843, respectively by affinity chromatography Sphingomonas elodea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-glucose + 2 NAD+ + H2O
-
Sphingomonas elodea UDP-glucuronic acid + 2 NADH + 2 H+
-
?
UDP-glucose + 2 NAD+ + H2O
-
Sphingomonas elodea ATCC 31461 UDP-glucuronic acid + 2 NADH + 2 H+
-
?

Synonyms

Synonyms Comment Organism
UDP-glucose dehydrogenase
-
Sphingomonas elodea
Ugd
-
Sphingomonas elodea
UgdG
-
Sphingomonas elodea

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Sphingomonas elodea

General Information

General Information Comment Organism
physiological function UDP-glucose dehydrogenase is responsible for the NAD-dependent twofold oxidation of UDP-glucose to UDP-glucuronic acid, one of the key components for gellan biosynthesis Sphingomonas elodea