Cloned (Comment) | Organism |
---|---|
gene ugdG, expression of His6-tagged wild-type and selenomethionine-labeled UgdG in Escherichia coli BL21 and B843, respectively | Sphingomonas elodea |
Crystallization (Comment) | Organism |
---|---|
purified recombinant wild-type and selenomethionine-labeled UgdG at 4.5 and 5.5 mg/ml, respectively, in 25 mM Tris-HCl, pH 8.3, 50 mM NaCl, 2.5 mM DTT and 1 mM NAD+, or 0.5 mM UDP-GlcA and 1 mM NAD+, 0.0005 ml of each protein and precipitant solution are mixed at 20°C, 24 h, the precipitant solution contains 200 mM Li2SO4, 100 mM Tris-HCl, pH 8.5, and 30% v/v PEG 4000, or 100 mM sodium citrate, pH 5.6, 20% 2-propanol, and 20% v/v PEG 4000, vapour diffusion method, method optimization, X-ray diffraction structure determination and analysis at 2.4 A resolution for the wild-type enzyme, and at 3.4 A resolution for the SeMet-UDG | Sphingomonas elodea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + 2 NAD+ + H2O | Sphingomonas elodea | - |
UDP-glucuronic acid + 2 NADH + 2 H+ | - |
? | |
UDP-glucose + 2 NAD+ + H2O | Sphingomonas elodea ATCC 31461 | - |
UDP-glucuronic acid + 2 NADH + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingomonas elodea | A4UTT2 | gene ugdG | - |
Sphingomonas elodea ATCC 31461 | A4UTT2 | gene ugdG | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged wild-type and selenomethionine-labeled UgdG from Escherichia coli BL21 and B843, respectively by affinity chromatography | Sphingomonas elodea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + 2 NAD+ + H2O | - |
Sphingomonas elodea | UDP-glucuronic acid + 2 NADH + 2 H+ | - |
? | |
UDP-glucose + 2 NAD+ + H2O | - |
Sphingomonas elodea ATCC 31461 | UDP-glucuronic acid + 2 NADH + 2 H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
UDP-glucose dehydrogenase | - |
Sphingomonas elodea |
Ugd | - |
Sphingomonas elodea |
UgdG | - |
Sphingomonas elodea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Sphingomonas elodea |
General Information | Comment | Organism |
---|---|---|
physiological function | UDP-glucose dehydrogenase is responsible for the NAD-dependent twofold oxidation of UDP-glucose to UDP-glucuronic acid, one of the key components for gellan biosynthesis | Sphingomonas elodea |