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Literature summary for 1.1.1.205 extracted from

  • Freedman, R.; Yu, R.; Sarkis, A.W.; Hedstrom, L.
    A structural determinant of mycophenolic acid resistance in eukaryotic inosine 5'-monophosphate dehydrogenases (2020), Protein Sci., 29, 686-694 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) DELTAguaB cells Aspergillus nidulans
expressed in Escherichia coli BL21(DE3) DELTAguaB cells Penicillium brevicompactum

Protein Variants

Protein Variants Comment Organism
A267S the mutant enzyme is inhibited by mycophenolic acid Aspergillus nidulans
S267A the mutant enzyme of isoform IMPDHA is inhibited by mycophenolic acid Penicillium brevicompactum

Inhibitors

Inhibitors Comment Organism Structure
additional information the enzyme is not inhibited by mycophenolic acid Aspergillus nidulans
additional information the enzyme is not inhibited by mycophenolic acid Penicillium brevicompactum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
IMP wild type enzyme, at pH 8.0 and 25°C Aspergillus nidulans
0.015
-
IMP mutant enzyme A267S, at pH 8.0 and 25°C Aspergillus nidulans
0.079
-
IMP mutant enzyme S267A of isoform IMPDHA, at pH 8.0 and 25°C Penicillium brevicompactum
0.093
-
IMP wild type enzyme isoform IMPDHA, at pH 8.0 and 25°C Penicillium brevicompactum
0.12
-
NAD+ wild type enzyme, at pH 8.0 and 25°C Aspergillus nidulans
0.19
-
NAD+ mutant enzyme A267S, at pH 8.0 and 25°C Aspergillus nidulans
0.33
-
NAD+ mutant enzyme S267A of isoform IMPDHA, at pH 8.0 and 25°C Penicillium brevicompactum
0.4
-
NAD+ wild type enzyme isoform IMPDHA, at pH 8.0 and 25°C Penicillium brevicompactum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
IMP + NAD+ + H2O Aspergillus nidulans
-
XMP + NADH + H+
-
?
IMP + NAD+ + H2O Penicillium brevicompactum
-
XMP + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus nidulans
-
-
-
Penicillium brevicompactum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
IMP + NAD+ + H2O
-
Aspergillus nidulans XMP + NADH + H+
-
?
IMP + NAD+ + H2O
-
Penicillium brevicompactum XMP + NADH + H+
-
?

Synonyms

Synonyms Comment Organism
IMPDH
-
Aspergillus nidulans
IMPDHA isoform Aspergillus nidulans
IMPDHA isoform Penicillium brevicompactum
inosine 5'-monophosphate dehydrogenase
-
Aspergillus nidulans
inosine 5'-monophosphate dehydrogenase
-
Penicillium brevicompactum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.6
-
NAD+ mutant enzyme S267A of isoform IMPDHA, at pH 8.0 and 25°C Penicillium brevicompactum
1.6
-
IMP mutant enzyme S267A of isoform IMPDHA, at pH 8.0 and 25°C Penicillium brevicompactum
1.9
-
NAD+ wild type enzyme, at pH 8.0 and 25°C Aspergillus nidulans
1.9
-
IMP wild type enzyme, at pH 8.0 and 25°C Aspergillus nidulans
1.9
-
NAD+ mutant enzyme A267S, at pH 8.0 and 25°C Aspergillus nidulans
1.9
-
IMP mutant enzyme A267S, at pH 8.0 and 25°C Aspergillus nidulans
2.7
-
NAD+ wild type enzyme isoform IMPDHA, at pH 8.0 and 25°C Penicillium brevicompactum
2.7
-
IMP wild type enzyme isoform IMPDHA, at pH 8.0 and 25°C Penicillium brevicompactum

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Aspergillus nidulans
NAD+
-
Penicillium brevicompactum