Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Ap4A | slight activation | Eremothecium gossypii | |
Ap5A | slight activation | Eremothecium gossypii | |
Ap6A | slight activation | Eremothecium gossypii | |
ATP | slight activation | Eremothecium gossypii |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Eremothecium gossypii |
Crystallization (Comment) | Organism |
---|---|
enzyme in complex with Ap5G and GDP, vapor diffusion method, using 20% (w/v) PEG-6000, 0.1 M sodium acetate, pH 5.0, 0.2 M sodium chloride | Eremothecium gossypii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GDP | - |
Eremothecium gossypii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 1 mM used in assay conditions | Eremothecium gossypii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
IMP + NAD+ + H2O | Eremothecium gossypii | - |
XMP + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Eremothecium gossypii | Q756Z6 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography and Sephacryl S-300 gel filtration | Eremothecium gossypii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
IMP + NAD+ + H2O | - |
Eremothecium gossypii | XMP + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | small angle X-ray scattering | Eremothecium gossypii |
Synonyms | Comment | Organism |
---|---|---|
IMPDH | - |
Eremothecium gossypii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Eremothecium gossypii |