Cloned (Comment) | Organism |
---|---|
gene IMH3, DNA sequence determination of wild-type and mutant IMH3, expression in enzyme-deficient Escherichia coli strain H712, subcloning in Escherichia coli strain DH5alpha | Candida albicans |
Protein Variants | Comment | Organism |
---|---|---|
A251T | the mutation affects the enzyme structure so that the mutant shows altered sensitivity to inhibitors compared to the wild-type enzyme | Candida albicans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | multiple inhibition with tiazofurin, competitive versus NAD+ | Candida albicans | |
mizoribine 5'-phosphate | competitive versus NAD+, slow tight-binding, the mutant A251T is 20fold more sesitive | Candida albicans | |
Mycophenolic acid | inhibition of the wild-type enzyme uncompetitively versus NAD+, the mutant A251T enzyme is 4fold more resistant to mycophenolic acid | Candida albicans | |
tiazofurin | multiple inhibition with ADP, noncompetitive versus NAD+ | Candida albicans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
inosine 5'-phosphate + NAD+ + H2O | Candida albicans | - |
xanthosine 5'-phosphate + NADH | - |
r | |
inosine 5'-phosphate + NAD+ + H2O | Candida albicans CAI-4 | - |
xanthosine 5'-phosphate + NADH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida albicans | Q59Q46 | strain CAI4, gene IMH3 | - |
Candida albicans CAI-4 | Q59Q46 | strain CAI4, gene IMH3 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from wild-type and mutant enzyme-deficient Escherichia coli strain H712 | Candida albicans |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
IMP + NAD+ + H2O = XMP + NADH + H+ | reaction mechanism | Candida albicans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
inosine 5'-phosphate + NAD+ + H2O | - |
Candida albicans | xanthosine 5'-phosphate + NADH | - |
r | |
inosine 5'-phosphate + NAD+ + H2O | - |
Candida albicans CAI-4 | xanthosine 5'-phosphate + NADH | - |
r |
Synonyms | Comment | Organism |
---|---|---|
IMPDH | - |
Candida albicans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Candida albicans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Candida albicans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Candida albicans | |
NADH | - |
Candida albicans |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics, structure-inhibitory potency relationship, overview | Candida albicans | |
0.00000001 | - |
mizoribine 5'-phosphate | pH 8.0, 25°C, recombinant mutant A251T | Candida albicans | |
0.0000002 | - |
mizoribine 5'-phosphate | pH 8.0, 25°C, recombinant wild-type enzyme | Candida albicans | |
0.0000011 | - |
Mycophenolic acid | pH 8.0, 25°C, recombinant wild-type enzyme | Candida albicans | |
0.0000044 | - |
Mycophenolic acid | pH 8.0, 25°C, recombinant mutant A251T | Candida albicans | |
16 | - |
ADP | pH 8.0, 25°C, recombinant mutant A251T | Candida albicans | |
26 | - |
tiazofurin | pH 8.0, 25°C, recombinant wild-type enzyme | Candida albicans | |
34 | - |
ADP | pH 8.0, 25°C, recombinant wild-type enzyme | Candida albicans | |
40 | - |
tiazofurin | pH 8.0, 25°C, recombinant mutant A251T | Candida albicans |