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Literature summary for 1.1.1.205 extracted from

  • Guillen Schlippe, Y.V.; Hedstrom, L.
    Guanidine derivatives rescue the Arg418Ala mutation of Tritrichomonas foetus IMP dehydrogenase (2005), Biochemistry, 44, 16695-16700.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in enzyme-deficient Escherichia coli strain H712 Tritrichomonas suis

Protein Variants

Protein Variants Comment Organism
R418A site-directed mutagenesis, altered kinetics compared to the wild-type enzyme, chemical rescue of the mutant by guanidine, methylguanidine, ethylguanidine, acteamide, and aminoguanidine, not by hydroxyurea, overview Tritrichomonas suis
R418A/Y419F site-directed mutagenesis, altered kinetics compared to the wild-type enzyme, no rescue of the mutant by aminoguanidine, overview Tritrichomonas suis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics, wild-type enzyme and mutant enzymes Tritrichomonas suis

Metals/Ions

Metals/Ions Comment Organism Structure
additional information 100-800 mM K+ does not affect the R418A mutant activity Tritrichomonas suis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
inosine 5'-phosphate + NAD+ + H2O Tritrichomonas suis
-
xanthosine 5'-phosphate + NADH
-
r

Organism

Organism UniProt Comment Textmining
Tritrichomonas suis
-
-
-

Reaction

Reaction Comment Organism Reaction ID
IMP + NAD+ + H2O = XMP + NADH + H+ Arg418 is involved in the reaction mechanism as catalytic base Tritrichomonas suis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
inosine 5'-phosphate + NAD+ + H2O
-
Tritrichomonas suis xanthosine 5'-phosphate + NADH
-
r

Synonyms

Synonyms Comment Organism
IMPDH
-
Tritrichomonas suis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Tritrichomonas suis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Tritrichomonas suis

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Tritrichomonas suis
NADH
-
Tritrichomonas suis