Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is not affected by allantoin, allantoic acid, uric acid, inosine, xanthosine, and xanthosine 5'-phosphate | Vigna unguiculata | |
Sulfhydryl-reducing agents | required for maximal activity | Vigna unguiculata |
General Stability | Organism |
---|---|
glycerol stabilizes | Vigna unguiculata |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | competitive | Vigna unguiculata | |
GMP | competitive | Vigna unguiculata | |
Mg2+ | inhibits K+ activation | Vigna unguiculata | |
additional information | the enzyme is not affected by allantoin, allantoic acid, uric acid, inosine, xanthosine, and xanthosine 5'-phosphate | Vigna unguiculata | |
NADH | - |
Vigna unguiculata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Vigna unguiculata | |
0.0091 | - |
inosine 5'-phosphate | pH 8.8, 30°C | Vigna unguiculata | |
0.018 | 0.035 | NAD+ | pH 8.8, 30°C | Vigna unguiculata |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Vigna unguiculata | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | activation | Vigna unguiculata | |
K+ | K+ activation is inhibited by Mg2+ | Vigna unguiculata | |
K+ | activates, Km: 1.6 mM, the activation is competitively inhibited by Mg2+ | Vigna unguiculata | |
additional information | no activation by NH4+ | Vigna unguiculata | |
NH4+ | no activation | Vigna unguiculata |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
SDS-PAGE | Vigna unguiculata |
200000 | - |
gel filtration | Vigna unguiculata |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
inosine 5'-phosphate + NAD+ + H2O | Vigna unguiculata | - |
xanthosine 5'-phosphate + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vigna unguiculata | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Vigna unguiculata |
native enzyme over 140fold from nitrogen-fixing nodules by ammonium sulfate fractionation, gel filtration, and affinity chromatography to homogeneity | Vigna unguiculata |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
nodule | nitrogen-fixing | Vigna unguiculata | - |
root nodule | nitrogen fixing nodules | Vigna unguiculata | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.1 | 1.5 | - |
Vigna unguiculata |
1.1 | 1.5 | purified enzyme | Vigna unguiculata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
inosine 5'-phosphate + NAD+ + H2O | - |
Vigna unguiculata | xanthosine 5'-phosphate + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 50000, SDS-PAGE | Vigna unguiculata |
Synonyms | Comment | Organism |
---|---|---|
IMP oxidoreductase | - |
Vigna unguiculata |
inosine monophosphate oxidoreductase | - |
Vigna unguiculata |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Vigna unguiculata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | - |
- |
Vigna unguiculata |
9.4 | - |
- |
Vigna unguiculata |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8.5 | 10 | pH 8.5: about 60% of activity maximum, pH 10: about 75% of activity maximum | Vigna unguiculata |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Vigna unguiculata | |
NAD+ | preferred cofactor, NADP+ shows poor activity, less that 10% compared to NAD+ | Vigna unguiculata | |
NADP+ | reduction less than 10% of that with NAD+ | Vigna unguiculata |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
GMP | - |
Vigna unguiculata | |
0.06 | - |
GMP | pH 8.8, 30°C | Vigna unguiculata |