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Literature summary for 1.1.1.205 extracted from

  • Atkins, C.A.; Shelp, B.J.; Storer, P.J.
    Purification and properties of inosine monophosphate oxidoreductase from nitrogen-fixing nodules of cowpea (Vigna unguiculata L. Walp) (1985), Arch. Biochem. Biophys., 236, 807-814.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information the enzyme is not affected by allantoin, allantoic acid, uric acid, inosine, xanthosine, and xanthosine 5'-phosphate Vigna unguiculata
Sulfhydryl-reducing agents required for maximal activity Vigna unguiculata

General Stability

General Stability Organism
glycerol stabilizes Vigna unguiculata

Inhibitors

Inhibitors Comment Organism Structure
AMP competitive Vigna unguiculata
GMP competitive Vigna unguiculata
Mg2+ inhibits K+ activation Vigna unguiculata
additional information the enzyme is not affected by allantoin, allantoic acid, uric acid, inosine, xanthosine, and xanthosine 5'-phosphate Vigna unguiculata
NADH
-
Vigna unguiculata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Vigna unguiculata
0.0091
-
inosine 5'-phosphate pH 8.8, 30°C Vigna unguiculata
0.018 0.035 NAD+ pH 8.8, 30°C Vigna unguiculata

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Vigna unguiculata
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ activation Vigna unguiculata
K+ K+ activation is inhibited by Mg2+ Vigna unguiculata
K+ activates, Km: 1.6 mM, the activation is competitively inhibited by Mg2+ Vigna unguiculata
additional information no activation by NH4+ Vigna unguiculata
NH4+ no activation Vigna unguiculata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
SDS-PAGE Vigna unguiculata
200000
-
gel filtration Vigna unguiculata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
inosine 5'-phosphate + NAD+ + H2O Vigna unguiculata
-
xanthosine 5'-phosphate + NADH
-
?

Organism

Organism UniProt Comment Textmining
Vigna unguiculata
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Vigna unguiculata
native enzyme over 140fold from nitrogen-fixing nodules by ammonium sulfate fractionation, gel filtration, and affinity chromatography to homogeneity Vigna unguiculata

Source Tissue

Source Tissue Comment Organism Textmining
nodule nitrogen-fixing Vigna unguiculata
-
root nodule nitrogen fixing nodules Vigna unguiculata
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1 1.5
-
Vigna unguiculata
1.1 1.5 purified enzyme Vigna unguiculata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
inosine 5'-phosphate + NAD+ + H2O
-
Vigna unguiculata xanthosine 5'-phosphate + NADH
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 50000, SDS-PAGE Vigna unguiculata

Synonyms

Synonyms Comment Organism
IMP oxidoreductase
-
Vigna unguiculata
inosine monophosphate oxidoreductase
-
Vigna unguiculata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Vigna unguiculata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8
-
-
Vigna unguiculata
9.4
-
-
Vigna unguiculata

pH Range

pH Minimum pH Maximum Comment Organism
8.5 10 pH 8.5: about 60% of activity maximum, pH 10: about 75% of activity maximum Vigna unguiculata

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Vigna unguiculata
NAD+ preferred cofactor, NADP+ shows poor activity, less that 10% compared to NAD+ Vigna unguiculata
NADP+ reduction less than 10% of that with NAD+ Vigna unguiculata

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.06
-
GMP
-
Vigna unguiculata
0.06
-
GMP pH 8.8, 30°C Vigna unguiculata