Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Agrobacterium tumefaciens |
Protein Variants | Comment | Organism |
---|---|---|
A39P | the mutant shows 103.5% activity compared to the wild type enzyme | Agrobacterium tumefaciens |
A39P/H234K | the mutant shows 165.7% activity compared to the wild type enzyme | Agrobacterium tumefaciens |
A39P/H99Y | the mutant shows 146.8% activity compared to the wild type enzyme | Agrobacterium tumefaciens |
A39P/H99Y/H234K | the triple mutant shows 247.6% activity compared to the wild type enzyme, a 400fold improvement in half-life at 59°C, and a 5°C improvement in T50 (10 min) value | Agrobacterium tumefaciens |
H234K | the mutant shows 129.4% activity compared to the wild type enzyme | Agrobacterium tumefaciens |
H99Y | the mutant shows 109.6% activity compared to the wild type enzyme | Agrobacterium tumefaciens |
H99Y/H234K | the mutant shows 175.4% activity compared to the wild type enzyme | Agrobacterium tumefaciens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
D-glucuronic acid | mutant enzyme A39P/H99Y/H234K, at pH 8.0 and 30°C | Agrobacterium tumefaciens | |
0.17 | - |
D-glucuronic acid | mutant enzyme H99Y/H234K, at pH 8.0 and 30°C | Agrobacterium tumefaciens | |
0.18 | - |
D-glucuronic acid | mutant enzyme A39P/H234K, at pH 8.0 and 30°C | Agrobacterium tumefaciens | |
0.2 | - |
D-glucuronic acid | wild type enzyme, at pH 8.0 and 30°C | Agrobacterium tumefaciens | |
0.2 | - |
D-glucuronic acid | mutant enzyme A39P/H99Y, at pH 8.0 and 30°C | Agrobacterium tumefaciens | |
0.23 | - |
D-glucuronic acid | mutant enzyme H99Y, at pH 8.0 and 30°C | Agrobacterium tumefaciens | |
0.24 | - |
D-glucuronic acid | mutant enzyme H234K, at pH 8.0 and 30°C | Agrobacterium tumefaciens | |
0.26 | - |
D-glucuronic acid | mutant enzyme A39P, at pH 8.0 and 30°C | Agrobacterium tumefaciens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galacturonic acid + NAD+ + H2O | Agrobacterium tumefaciens | - |
D-galactaric acid + NADH + H+ | - |
? | |
D-galacturonic acid + NAD+ + H2O | Agrobacterium tumefaciens LBA4404 | - |
D-galactaric acid + NADH + H+ | - |
? | |
D-glucuronic acid + NAD+ + H2O | Agrobacterium tumefaciens | - |
D-glucaric acid + NADH + H+ | - |
? | |
D-glucuronic acid + NAD+ + H2O | Agrobacterium tumefaciens LBA4404 | - |
D-glucaric acid + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agrobacterium tumefaciens | - |
- |
- |
Agrobacterium tumefaciens LBA4404 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
85 | - |
wild type enzyme, at pH 8.0 and 30°C | Agrobacterium tumefaciens |
88 | - |
mutant enzyme A39P, at pH 8.0 and 30°C | Agrobacterium tumefaciens |
93 | - |
mutant enzyme H99Y, at pH 8.0 and 30°C | Agrobacterium tumefaciens |
110 | - |
mutant enzyme H234K, at pH 8.0 and 30°C | Agrobacterium tumefaciens |
124 | - |
mutant enzyme A39P/H99Y, at pH 8.0 and 30°C | Agrobacterium tumefaciens |
140 | - |
mutant enzyme A39P/H234K, at pH 8.0 and 30°C | Agrobacterium tumefaciens |
149 | - |
mutant enzyme H99Y/H234K, at pH 8.0 and 30°C | Agrobacterium tumefaciens |
210 | - |
mutant enzyme A39P/H99Y/H234K, at pH 8.0 and 30°C | Agrobacterium tumefaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galacturonic acid + NAD+ + H2O | - |
Agrobacterium tumefaciens | D-galactaric acid + NADH + H+ | - |
? | |
D-galacturonic acid + NAD+ + H2O | - |
Agrobacterium tumefaciens LBA4404 | D-galactaric acid + NADH + H+ | - |
? | |
D-glucuronic acid + NAD+ + H2O | - |
Agrobacterium tumefaciens | D-glucaric acid + NADH + H+ | - |
? | |
D-glucuronic acid + NAD+ + H2O | - |
Agrobacterium tumefaciens LBA4404 | D-glucaric acid + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 32000, SDS-PAGE | Agrobacterium tumefaciens |
Synonyms | Comment | Organism |
---|---|---|
Udh | - |
Agrobacterium tumefaciens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Agrobacterium tumefaciens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
59 | - |
the enzyme has a half-life of 0.3 min at 59°C (pH 8.0) | Agrobacterium tumefaciens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
161 | - |
D-glucuronic acid | wild type enzyme, at pH 8.0 and 30°C | Agrobacterium tumefaciens | |
179 | - |
D-glucuronic acid | mutant enzyme H99Y, at pH 8.0 and 30°C | Agrobacterium tumefaciens | |
192 | - |
D-glucuronic acid | mutant enzyme A39P, at pH 8.0 and 30°C | Agrobacterium tumefaciens | |
195 | - |
D-glucuronic acid | mutant enzyme H234K, at pH 8.0 and 30°C | Agrobacterium tumefaciens | |
239 | - |
D-glucuronic acid | mutant enzyme A39P/H99Y, at pH 8.0 and 30°C | Agrobacterium tumefaciens | |
295 | - |
D-glucuronic acid | mutant enzyme A39P/H234K, at pH 8.0 and 30°C | Agrobacterium tumefaciens | |
318 | - |
D-glucuronic acid | mutant enzyme H99Y/H234K, at pH 8.0 and 30°C | Agrobacterium tumefaciens | |
380 | - |
D-glucuronic acid | mutant enzyme A39P/H99Y/H234K, at pH 8.0 and 30°C | Agrobacterium tumefaciens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Agrobacterium tumefaciens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Agrobacterium tumefaciens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
720 | - |
D-glucuronic acid | mutant enzyme A39P/H99Y, at pH 8.0 and 30°C | Agrobacterium tumefaciens | |
730 | - |
D-glucuronic acid | mutant enzyme A39P, at pH 8.0 and 30°C | Agrobacterium tumefaciens | |
770 | - |
D-glucuronic acid | mutant enzyme H99Y, at pH 8.0 and 30°C | Agrobacterium tumefaciens | |
800 | - |
D-glucuronic acid | wild type enzyme, at pH 8.0 and 30°C | Agrobacterium tumefaciens | |
810 | - |
D-glucuronic acid | mutant enzyme H234K, at pH 8.0 and 30°C | Agrobacterium tumefaciens | |
840 | - |
D-glucuronic acid | mutant enzyme A39P/H234K, at pH 8.0 and 30°C | Agrobacterium tumefaciens | |
860 | - |
D-glucuronic acid | mutant enzyme H99Y/H234K, at pH 8.0 and 30°C | Agrobacterium tumefaciens | |
910 | - |
D-glucuronic acid | mutant enzyme A39P/H99Y/H234K, at pH 8.0 and 30°C | Agrobacterium tumefaciens |