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Literature summary for 1.1.1.203 extracted from

  • Su, H.; Peng, F.; Xu, P.; Wu, X.; Zong, M.; Yang, J.; Lou, W.
    Enhancing the thermostability and activity of uronate dehydrogenase from Agrobacterium tumefaciens LBA4404 by semi-rational engineering (2019), Bioresour. Bioprocess., 6, 36 .
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Agrobacterium tumefaciens

Protein Variants

Protein Variants Comment Organism
A39P the mutant shows 103.5% activity compared to the wild type enzyme Agrobacterium tumefaciens
A39P/H234K the mutant shows 165.7% activity compared to the wild type enzyme Agrobacterium tumefaciens
A39P/H99Y the mutant shows 146.8% activity compared to the wild type enzyme Agrobacterium tumefaciens
A39P/H99Y/H234K the triple mutant shows 247.6% activity compared to the wild type enzyme, a 400fold improvement in half-life at 59°C, and a 5°C improvement in T50 (10 min) value Agrobacterium tumefaciens
H234K the mutant shows 129.4% activity compared to the wild type enzyme Agrobacterium tumefaciens
H99Y the mutant shows 109.6% activity compared to the wild type enzyme Agrobacterium tumefaciens
H99Y/H234K the mutant shows 175.4% activity compared to the wild type enzyme Agrobacterium tumefaciens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
D-glucuronic acid mutant enzyme A39P/H99Y/H234K, at pH 8.0 and 30°C Agrobacterium tumefaciens
0.17
-
D-glucuronic acid mutant enzyme H99Y/H234K, at pH 8.0 and 30°C Agrobacterium tumefaciens
0.18
-
D-glucuronic acid mutant enzyme A39P/H234K, at pH 8.0 and 30°C Agrobacterium tumefaciens
0.2
-
D-glucuronic acid wild type enzyme, at pH 8.0 and 30°C Agrobacterium tumefaciens
0.2
-
D-glucuronic acid mutant enzyme A39P/H99Y, at pH 8.0 and 30°C Agrobacterium tumefaciens
0.23
-
D-glucuronic acid mutant enzyme H99Y, at pH 8.0 and 30°C Agrobacterium tumefaciens
0.24
-
D-glucuronic acid mutant enzyme H234K, at pH 8.0 and 30°C Agrobacterium tumefaciens
0.26
-
D-glucuronic acid mutant enzyme A39P, at pH 8.0 and 30°C Agrobacterium tumefaciens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-galacturonic acid + NAD+ + H2O Agrobacterium tumefaciens
-
D-galactaric acid + NADH + H+
-
?
D-galacturonic acid + NAD+ + H2O Agrobacterium tumefaciens LBA4404
-
D-galactaric acid + NADH + H+
-
?
D-glucuronic acid + NAD+ + H2O Agrobacterium tumefaciens
-
D-glucaric acid + NADH + H+
-
?
D-glucuronic acid + NAD+ + H2O Agrobacterium tumefaciens LBA4404
-
D-glucaric acid + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Agrobacterium tumefaciens
-
-
-
Agrobacterium tumefaciens LBA4404
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
85
-
wild type enzyme, at pH 8.0 and 30°C Agrobacterium tumefaciens
88
-
mutant enzyme A39P, at pH 8.0 and 30°C Agrobacterium tumefaciens
93
-
mutant enzyme H99Y, at pH 8.0 and 30°C Agrobacterium tumefaciens
110
-
mutant enzyme H234K, at pH 8.0 and 30°C Agrobacterium tumefaciens
124
-
mutant enzyme A39P/H99Y, at pH 8.0 and 30°C Agrobacterium tumefaciens
140
-
mutant enzyme A39P/H234K, at pH 8.0 and 30°C Agrobacterium tumefaciens
149
-
mutant enzyme H99Y/H234K, at pH 8.0 and 30°C Agrobacterium tumefaciens
210
-
mutant enzyme A39P/H99Y/H234K, at pH 8.0 and 30°C Agrobacterium tumefaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galacturonic acid + NAD+ + H2O
-
Agrobacterium tumefaciens D-galactaric acid + NADH + H+
-
?
D-galacturonic acid + NAD+ + H2O
-
Agrobacterium tumefaciens LBA4404 D-galactaric acid + NADH + H+
-
?
D-glucuronic acid + NAD+ + H2O
-
Agrobacterium tumefaciens D-glucaric acid + NADH + H+
-
?
D-glucuronic acid + NAD+ + H2O
-
Agrobacterium tumefaciens LBA4404 D-glucaric acid + NADH + H+
-
?

Subunits

Subunits Comment Organism
? x * 32000, SDS-PAGE Agrobacterium tumefaciens

Synonyms

Synonyms Comment Organism
Udh
-
Agrobacterium tumefaciens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Agrobacterium tumefaciens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
59
-
the enzyme has a half-life of 0.3 min at 59°C (pH 8.0) Agrobacterium tumefaciens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
161
-
D-glucuronic acid wild type enzyme, at pH 8.0 and 30°C Agrobacterium tumefaciens
179
-
D-glucuronic acid mutant enzyme H99Y, at pH 8.0 and 30°C Agrobacterium tumefaciens
192
-
D-glucuronic acid mutant enzyme A39P, at pH 8.0 and 30°C Agrobacterium tumefaciens
195
-
D-glucuronic acid mutant enzyme H234K, at pH 8.0 and 30°C Agrobacterium tumefaciens
239
-
D-glucuronic acid mutant enzyme A39P/H99Y, at pH 8.0 and 30°C Agrobacterium tumefaciens
295
-
D-glucuronic acid mutant enzyme A39P/H234K, at pH 8.0 and 30°C Agrobacterium tumefaciens
318
-
D-glucuronic acid mutant enzyme H99Y/H234K, at pH 8.0 and 30°C Agrobacterium tumefaciens
380
-
D-glucuronic acid mutant enzyme A39P/H99Y/H234K, at pH 8.0 and 30°C Agrobacterium tumefaciens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Agrobacterium tumefaciens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Agrobacterium tumefaciens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
720
-
D-glucuronic acid mutant enzyme A39P/H99Y, at pH 8.0 and 30°C Agrobacterium tumefaciens
730
-
D-glucuronic acid mutant enzyme A39P, at pH 8.0 and 30°C Agrobacterium tumefaciens
770
-
D-glucuronic acid mutant enzyme H99Y, at pH 8.0 and 30°C Agrobacterium tumefaciens
800
-
D-glucuronic acid wild type enzyme, at pH 8.0 and 30°C Agrobacterium tumefaciens
810
-
D-glucuronic acid mutant enzyme H234K, at pH 8.0 and 30°C Agrobacterium tumefaciens
840
-
D-glucuronic acid mutant enzyme A39P/H234K, at pH 8.0 and 30°C Agrobacterium tumefaciens
860
-
D-glucuronic acid mutant enzyme H99Y/H234K, at pH 8.0 and 30°C Agrobacterium tumefaciens
910
-
D-glucuronic acid mutant enzyme A39P/H99Y/H234K, at pH 8.0 and 30°C Agrobacterium tumefaciens