Application | Comment | Organism |
---|---|---|
food industry | mequindox, inhibitor of several Gram-positive and Gram-negative bacteria, is reduced by carbonyl reductase CBR1 | Sus scrofa |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Sus scrofa |
expression in Escherichia coli | Gallus gallus |
Protein Variants | Comment | Organism |
---|---|---|
E142M | increase in reaction velocity | Sus scrofa |
E142V | increase in reaction velocity | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0199 | - |
menadione | pH 7.4, 37°C | Sus scrofa | |
0.076 | - |
menadione | pH 7.4, 37°C | Gallus gallus | |
0.105 | - |
1-(3-methyl-4-oxidoquinoxalin-2-yl)ethanone | pH 7.4, 37°C | Gallus gallus | |
0.116 | - |
1-(3-methyl-4-oxidoquinoxalin-2-yl)ethanone | pH 7.4, 37°C | Sus scrofa | |
0.156 | - |
1-(3-methyl-1,4-dioxidoquinoxalin-2-yl)ethanone | pH 7.4, 37°C | Gallus gallus | |
0.469 | - |
1-(3-methyl-1,4-dioxidoquinoxalin-2-yl)ethanone | pH 7.4, 37°C | Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Sus scrofa | 5829 | - |
cytosol | - |
Gallus gallus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
x * 31000, SDS-PAGE, x * 31590, calculated, His-tagged protein | Sus scrofa |
31000 | - |
x * 31000, SDS-PAGE, x * 31590, calculated, His-tagged protein | Gallus gallus |
31590 | - |
x * 31000, SDS-PAGE, x * 31590, calculated, His-tagged protein | Sus scrofa |
31590 | - |
x * 31000, SDS-PAGE, x * 31590, calculated, His-tagged protein | Gallus gallus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | F1N8Y3 | putative | - |
Sus scrofa | Q28960 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Sus scrofa | - |
liver | - |
Gallus gallus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-(3-methyl-1,4-dioxidoquinoxalin-2-yl)ethanone + NADPH + H+ | i.e. mequindox, synthetic quinoxaline derivative, inhibitory to several Gram-positive and Gram-negative bacteria | Sus scrofa | 1-(3-methyl-1,4-dioxidoquinoxalin-2-yl)ethanol + NADP+ | - |
? | |
1-(3-methyl-1,4-dioxidoquinoxalin-2-yl)ethanone + NADPH + H+ | i.e. mequindox, synthetic quinoxaline derivative, inhibitory to several Gram-positive and Gram-negative bactria | Gallus gallus | 1-(3-methyl-1,4-dioxidoquinoxalin-2-yl)ethanol + NADP+ | - |
? | |
1-(3-methyl-4-oxidoquinoxalin-2-yl)ethanone + NADPH + H+ | - |
Sus scrofa | 1-(3-methyl-4-oxidoquinoxalin-2-yl)ethanol + NADP+ | - |
? | |
1-(3-methyl-4-oxidoquinoxalin-2-yl)ethanone + NADPH + H+ | - |
Gallus gallus | 1-(3-methyl-4-oxidoquinoxalin-2-yl)ethanol + NADP+ | - |
? | |
menadione + NADPH + H+ | - |
Sus scrofa | ? + NADP+ | - |
? | |
menadione + NADPH + H+ | - |
Gallus gallus | ? + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 31000, SDS-PAGE, x * 31590, calculated, His-tagged protein | Sus scrofa |
? | x * 31000, SDS-PAGE, x * 31590, calculated, His-tagged protein | Gallus gallus |
Synonyms | Comment | Organism |
---|---|---|
CBR1 | - |
Sus scrofa |
CBR1 | - |
Gallus gallus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00027 | - |
1-(3-methyl-1,4-dioxidoquinoxalin-2-yl)ethanone | pH 7.4, 37°C | Sus scrofa | |
0.00062 | - |
1-(3-methyl-1,4-dioxidoquinoxalin-2-yl)ethanone | pH 7.4, 37°C | Gallus gallus | |
0.15 | - |
menadione | pH 7.4, 37°C | Sus scrofa | |
2.72 | - |
1-(3-methyl-4-oxidoquinoxalin-2-yl)ethanone | pH 7.4, 37°C | Gallus gallus | |
2.89 | - |
1-(3-methyl-4-oxidoquinoxalin-2-yl)ethanone | pH 7.4, 37°C | Sus scrofa | |
4.17 | - |
menadione | pH 7.4, 37°C | Gallus gallus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4 | - |
1-(3-methyl-1,4-dioxidoquinoxalin-2-yl)ethanone | pH 7.4, 37°C | Gallus gallus | |
5.75 | - |
1-(3-methyl-1,4-dioxidoquinoxalin-2-yl)ethanone | pH 7.4, 37°C | Sus scrofa | |
7.5 | - |
menadione | pH 7.4, 37°C | Sus scrofa | |
25 | - |
1-(3-methyl-4-oxidoquinoxalin-2-yl)ethanone | pH 7.4, 37°C | Sus scrofa | |
26 | - |
1-(3-methyl-4-oxidoquinoxalin-2-yl)ethanone | pH 7.4, 37°C | Gallus gallus | |
54.5 | - |
menadione | pH 7.4, 37°C | Gallus gallus |