Cloned (Comment) | Organism |
---|---|
expression of soluble, active enzyme as GST-fusion enzyme in Escherichia coli | Homo sapiens |
expression of soluble, active enzyme as GST-fusion enzyme in Escherichia coli | Oryctolagus cuniculus |
Crystallization (Comment) | Organism |
---|---|
in complex with NADP+ and progesterone | Homo sapiens |
in complex with NADP+ and progesterone, with NADP+ and dihydrotestosterone, with NADP+ and 4-androstenedione | Oryctolagus cuniculus |
purified recombinant enzyme in complex with cofactor and several substrates, hanging drop vapour diffusion method, 4°C, HEPES or sodium acetate buffer, CaCl2, precipitant is PEG 4000, X-ray diffraction structure determination and analysis at 1.7-1.9 A resolution | Oryctolagus cuniculus |
purified recombinant enzyme in complex with cofactor and several substrates, hanging drop vapour diffusion method, 4°C, HEPES or sodium acetate buffer, CaCl2, precipitant is PEG 4000, X-ray diffraction structure determination and analysis at 2.4-2.5 A resolution | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
17alpha-hydroxyprogesterone + NAD(P)H | Homo sapiens | - |
17alpha,20alpha-dihydroxypregn-4-en-3-one + NAD(P)+ | - |
r | |
17alpha-hydroxyprogesterone + NAD(P)H | Oryctolagus cuniculus | - |
17alpha,20alpha-dihydroxypregn-4-en-3-one + NAD(P)+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST-fusion enzyme by glutahione affinity chromatography and cleavage of the GST-tag by Factor Xa, followed by ion exchange chromatography, and gel filtration, to homogeneity | Homo sapiens |
recombinant GST-fusion enzyme by glutahione affinity chromatography and cleavage of the GST-tag by Factor Xa, followed by ion exchange chromatography, and gel filtration, to homogeneity | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
17alpha-hydroxyprogesterone + NAD(P)H | - |
Homo sapiens | 17alpha,20alpha-dihydroxypregn-4-en-3-one + NAD(P)+ | - |
r | |
17alpha-hydroxyprogesterone + NAD(P)H | - |
Oryctolagus cuniculus | 17alpha,20alpha-dihydroxypregn-4-en-3-one + NAD(P)+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
20alpha-HSD | - |
Homo sapiens |
20alpha-HSD | - |
Oryctolagus cuniculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
37 | - |
assay at | Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
7.5 | - |
assay at | Oryctolagus cuniculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD(P)+ | - |
Homo sapiens | |
NAD(P)+ | - |
Oryctolagus cuniculus | |
NAD(P)H | - |
Homo sapiens | |
NAD(P)H | - |
Oryctolagus cuniculus |